Alignments for a candidate for lacZ in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacZ in Herbaspirillum seropedicae SmR1

Align Beta-galactosidase BgaT; Beta-gal; EC 3.2.1.23 (characterized)
to candidate HSERO_RS01350 HSERO_RS01350 beta-galactosidase

Query= SwissProt::Q9X6C6
         (645 letters)



>FitnessBrowser__HerbieS:HSERO_RS01350
          Length = 658

 Score =  712 bits (1838), Expect = 0.0
 Identities = 350/654 (53%), Positives = 441/654 (67%), Gaps = 13/654 (1%)

Query: 2   LGVCYYPEHWPRERWSEDARRMRELGLAYVRVGEFAWALLEPEPGRLDWAWLDEAVAVLA 61
           LGVCYYPEHWP   W  DA+RM+ LG+  VR+GEFAW+ +EP PG L W WLD A+ VLA
Sbjct: 5   LGVCYYPEHWPEAMWDSDAQRMKALGIKQVRIGEFAWSRIEPSPGELRWDWLDRAIEVLA 64

Query: 62  QAGLKVVLGTPTATPPKWLVDRYPEILPVDREGRRRRFGGRRHYCFSSPVYHEETRRIVT 121
           + GL+VV+ TPTATPPKWL+DR+ ++L VD  GR+R FG RRHY FSS  Y EE++RIV 
Sbjct: 65  RHGLEVVMCTPTATPPKWLIDRHDDVLAVDANGRQRAFGSRRHYDFSSDSYFEESQRIVR 124

Query: 122 LLGERYGKHPAVAGFQTDNEYGCHGTVRCYCERCQDAFRKWLEERYGSIDVLNEAWGTVF 181
           LLGERYG+HPAV  +QTDNEYGCH TV  Y    Q  FR+WL +RYGSID LN AWGTVF
Sbjct: 125 LLGERYGQHPAVTAWQTDNEYGCHQTVVSYSASAQRRFRQWLRQRYGSIDALNTAWGTVF 184

Query: 182 WSQRYRTFQEVELPNLTVAEANPSHLLDYYRFASEQVRRYNRLQVEILRAHAPGKFVTHN 241
           WS  YR+F E++ P  TV EA+PSH LDY RFAS++V RYNR+QVEILRA +PG+ + HN
Sbjct: 185 WSMEYRSFDEIDAPIGTVTEAHPSHRLDYRRFASDEVARYNRMQVEILRALSPGRLMVHN 244

Query: 242 FMGFFTDLNPFPLGEDLDFASWDSYPLGFTDLMPLPEEEKLRYARTGHPDVAAFHHDLYR 301
           FM  F + + +P+  DLD A+WDSYPLG  ++M   +  K R+ RTGHPD A+F+HDLYR
Sbjct: 245 FMQMFLEFDHYPVAADLDVATWDSYPLGALEVMWFDDATKARWLRTGHPDFASFNHDLYR 304

Query: 302 AVGRGRFWVMEQQPGPVNWAPHNPNPAPGMVRLWTWEALANGAEVVSYFRWRQVPFAQEQ 361
            +    FWVMEQQPGPVNWA  NP+P PGMVRLW+WEA A+GA  VSYFRWRQ PFAQEQ
Sbjct: 305 GMSAQPFWVMEQQPGPVNWAHWNPHPLPGMVRLWSWEAFAHGAGCVSYFRWRQCPFAQEQ 364

Query: 362 MHAGLHRPDYAPDAAFFEVQRVVEEL-------GALSLPPPGQAPVALVYDPEAPWVYEV 414
           +HAGL+RPD   D    E ++V  E+       GAL+ P   +  VAL++D +A W++++
Sbjct: 365 LHAGLNRPDNRLDVGGSEAEQVAREIITVEQAQGALAQP---RGKVALLFDYDAKWLFDI 421

Query: 415 QPHGAEWNYLALVFLFYSVARRLGLDVDIVPPGAALQGYRLVLVPSLPIVREKALNAFRE 474
              G ++ Y    F +Y+  R LGLDVDI+P  A L GY +++VP LPIV +       E
Sbjct: 422 HFQGIDFEYQRFAFQYYATLRALGLDVDILPLHAKLDGYAMIVVPPLPIVPDDLPGRIAE 481

Query: 475 ADGIVLFGPRSGSKNENFHIPQGLPPGPLQALLPLKVVRVESLPPGLREEVEGPWGRFSS 534
           +  + +FGPRSGSK  +  IP  LPPGPLQ LLP++V RVESL P + E V+        
Sbjct: 482 SGALSVFGPRSGSKTRSLQIPATLPPGPLQQLLPMRVWRVESLRPNVTEPVQFGAHSGQG 541

Query: 535 GLWREWVETD---LSPLLRFADGLGALFRAGRYLYLAAWPSPELLGALLVGLAQEGGLSP 591
             WR+ +E D   L  L +F DG  A+ R  R  YLA      L  A    LA++ GL+P
Sbjct: 542 AHWRDLIEADGEGLQTLAQFGDGHPAIVRKERAHYLAGIFDDALTSACFEQLARDAGLAP 601

Query: 592 KPLPSGLRLRWRGHLVFAFNYGPEEVVLPVPSGVRFRLGGPRLSPYEVAVWEEG 645
           + LP GLRL+ RG L FAFNY    + LP   G +F +G  +L P  VAV+  G
Sbjct: 602 QRLPEGLRLQERGGLCFAFNYSDAAITLPQAQGAQFLVGQAQLEPQGVAVYRTG 655


Lambda     K      H
   0.322    0.142    0.469 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1542
Number of extensions: 80
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 645
Length of database: 658
Length adjustment: 38
Effective length of query: 607
Effective length of database: 620
Effective search space:   376340
Effective search space used:   376340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources