GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hisP in Herbaspirillum seropedicae SmR1

Align BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized)
to candidate HSERO_RS00320 HSERO_RS00320 peptide ABC transporter ATP-binding protein

Query= TCDB::P73721
         (252 letters)



>lcl|FitnessBrowser__HerbieS:HSERO_RS00320 HSERO_RS00320 peptide ABC
           transporter ATP-binding protein
          Length = 240

 Score =  278 bits (710), Expect = 9e-80
 Identities = 143/242 (59%), Positives = 180/242 (74%), Gaps = 3/242 (1%)

Query: 8   LISFDQLQKNFGALQVLRGVTGEIYPKDVISIIGPSGCGKSTFLRCLNRLEPISGGRLEV 67
           +I  +QLQK FG   +LRG+  EI  ++V+ +IGPSG GKSTFLRCLN LE +S G + +
Sbjct: 1   MIKVNQLQKRFGQAHILRGIDCEIRAREVVCVIGPSGSGKSTFLRCLNGLEEVSDGDIFI 60

Query: 68  AGVDLSGAKIDQKHLRQLRVRVGMVFQHFNLFPHLTVLQNLLLAPRKVLRIPMAEAKDRA 127
            GV L+  K++   L  LR  +GMVFQ FNLFPH+TVL+NL++AP +V ++   EA   A
Sbjct: 61  EGVKLNDPKVN---LNALRAELGMVFQRFNLFPHMTVLENLIMAPMQVKKLSRREAVLVA 117

Query: 128 LTYLDKVGLGTKADNYPDQLSGGQKQRVAIARGLCMKPEILLFDEPTSALDPELVGEVLN 187
              L KVGL  K D +P+QLSGGQ+QRVAIAR L M+P+++LFDEPTSALDPELVGEVL 
Sbjct: 118 EKLLQKVGLLDKIDAFPNQLSGGQQQRVAIARALAMEPKVMLFDEPTSALDPELVGEVLT 177

Query: 188 VMKQLAEEGMTMAVVTHEMQFAREVSNRVFFFNQGIIEEEGDPNEVFRNPKSDRLRAFLS 247
           VMKQLAEEGMTM VVTHEM FAREVS+RV F +QG+I EEG P +V  +P  +R R FL 
Sbjct: 178 VMKQLAEEGMTMVVVTHEMGFAREVSDRVLFIDQGVIMEEGPPQQVLGDPHHERTRDFLR 237

Query: 248 RI 249
           ++
Sbjct: 238 KV 239


Lambda     K      H
   0.321    0.139    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 211
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 252
Length of database: 240
Length adjustment: 24
Effective length of query: 228
Effective length of database: 216
Effective search space:    49248
Effective search space used:    49248
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory