Alignments for a candidate for malK_Ss in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for malK_Ss in Herbaspirillum seropedicae SmR1

Align MalK, component of Maltose and maltooligosaccharide porter (characterized)
to candidate HSERO_RS15940 HSERO_RS15940 peptide ABC transporter substrate-binding protein

Query= TCDB::Q97UG5
         (617 letters)



>FitnessBrowser__HerbieS:HSERO_RS15940
          Length = 334

 Score =  190 bits (483), Expect = 7e-53
 Identities = 111/322 (34%), Positives = 181/322 (56%), Gaps = 10/322 (3%)

Query: 5   MDSLLKVNELTAGYFNQDGFVIGVTNVNFEVYPNEIFAIAGESGCGKSTLAMAIYGLLKY 64
           M  LL V  L      ++G +  V  ++F+V   E+  + GESGCGKS  ++A+ GLL  
Sbjct: 1   MSVLLDVKNLKVDLPTENGMLHAVRGIDFQVRRGEMLCLVGESGCGKSMTSLALMGLLPR 60

Query: 65  PGVVLRGHVYLKDKDILSITQEELRKLRMKEFVYVPQFAMDALDPVAKIGDQMMRAAVSH 124
                  H+     D+  +  ++L +LR K    + Q  M +L+P   +G+Q+  A +  
Sbjct: 61  KAQCSADHILFDGVDLHGMPDKQLMQLRGKRMAMIFQEPMTSLNPSYTLGNQLCEAMLQQ 120

Query: 125 -GVNVEEARKLIKEKLELVDLPY--NVVNMYPHELSGGMRQRVVIATSILLNPSLIILDE 181
            GV+  EAR+     L    +    + +  YPH+LSGG+RQRV+IA S++ NP LII DE
Sbjct: 121 PGVSRAEARERALYLLHRTGISNAEDRLRQYPHQLSGGLRQRVMIAMSLMCNPDLIIADE 180

Query: 182 PTTGLDVIVQYEILKDLKRIQRQLGVSLVIISHDISMLLMISDRVGIMYAGEIVEIGSKE 241
           PTT LDV +Q +IL+ ++ +Q++ G +++ I+HD+ ++  I+DRV +MYAG++VE     
Sbjct: 181 PTTALDVTIQAQILRMIRELQQEFGAAVIFITHDLGVVSRIADRVAVMYAGQVVETTDVA 240

Query: 242 EIIKRPSHPYTYLLISSLPSLVKR--REKLLSIPGNPPLMLSKVPNSCRFYDRCPFKMEK 299
           ++  +P HPYT  L++ +P   K      L +IPG  P ++ +V + C F +RC  K + 
Sbjct: 241 QLFAQPRHPYTQGLLNCIPVRGKTLPGSHLQAIPGVVPSLVGQV-SGCAFRNRCA-KADS 298

Query: 300 CSTLNPAL---GDIMDGHKARC 318
               +P +   G +   H+ARC
Sbjct: 299 GCERDPQMVQQGSVTAPHQARC 320



 Score =  144 bits (363), Expect = 6e-39
 Identities = 85/259 (32%), Positives = 151/259 (58%), Gaps = 12/259 (4%)

Query: 370 KNLILSEP-----INAVNDVSFELKKGTITALVGGSGHGKSTIAKILAGMI----QQTSG 420
           KNL +  P     ++AV  + F++++G +  LVG SG GKS  +  L G++    Q ++ 
Sbjct: 8   KNLKVDLPTENGMLHAVRGIDFQVRRGEMLCLVGESGCGKSMTSLALMGLLPRKAQCSAD 67

Query: 421 KIILLGKDVSEYGVRNSMWYK-ENVQMIFQDPYSSLDPRHTVRWHVERPLLIHKKVSNKD 479
            I+  G D+     +  M  + + + MIFQ+P +SL+P +T+   +   +L    VS + 
Sbjct: 68  HILFDGVDLHGMPDKQLMQLRGKRMAMIFQEPMTSLNPSYTLGNQLCEAMLQQPGVS-RA 126

Query: 480 QLLPKIIEVLKNVGLKPPEKYLYKYPHELSGGERQRVAIARATAVEPKVLVADEPVSMLD 539
           +   + + +L   G+   E  L +YPH+LSGG RQRV IA +    P +++ADEP + LD
Sbjct: 127 EARERALYLLHRTGISNAEDRLRQYPHQLSGGLRQRVMIAMSLMCNPDLIIADEPTTALD 186

Query: 540 ASLRAGILNLIKKFKKN-GISILYITHDIATVNYIADEIMVIYKGRIVEKGNTYEVISNP 598
            +++A IL +I++ ++  G ++++ITHD+  V+ IAD + V+Y G++VE  +  ++ + P
Sbjct: 187 VTIQAQILRMIRELQQEFGAAVIFITHDLGVVSRIADRVAVMYAGQVVETTDVAQLFAQP 246

Query: 599 SHEYTKRLIEAVPDPYKTI 617
            H YT+ L+  +P   KT+
Sbjct: 247 RHPYTQGLLNCIPVRGKTL 265


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 425
Number of extensions: 23
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 617
Length of database: 334
Length adjustment: 33
Effective length of query: 584
Effective length of database: 301
Effective search space:   175784
Effective search space used:   175784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources