Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate HSERO_RS05320 HSERO_RS05320 ribonucleotide-diphosphate reductase subunit alpha
Query= uniprot:D8IZC7 (521 letters) >FitnessBrowser__HerbieS:HSERO_RS05320 Length = 502 Score = 409 bits (1052), Expect = e-118 Identities = 222/503 (44%), Positives = 312/503 (62%), Gaps = 3/503 (0%) Query: 6 LLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILL 65 +LQ+ GI+K+FG L + L +R GE+HAL+GENGAGKSTLMK+L G+ PD GEI + Sbjct: 1 MLQLTGIKKNFGPVTVLRGVDLEVRAGEVHALLGENGAGKSTLMKILCGIVRPDAGEIRI 60 Query: 66 DGRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTD 125 DG+P A+ A G+ +++QE ++ P + N+F+ ELR+R G + AAMR R Sbjct: 61 DGQPCRFDSYRAAIAGGVGVVFQEFSLIPYLDAVDNMFLARELRSRWGWLQRAAMRRRAQ 120 Query: 126 AVLRQLGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNV 185 ++ QLG +LS+A+QQ VEIA+AL +RI+++DEPTA L+ E E LF V Sbjct: 121 EIIGQLGVAIPLDVPVCKLSVAQQQFVEIAKALALDARILVLDEPTATLTPAEVEHLFAV 180 Query: 186 VRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRSLS 245 +R LR +G+AII+ISH + E++ + DR+TVLRDG++V E+D R+V+MMVGR + Sbjct: 181 MRSLRAQGVAIIFISHHLEEIFEICDRITVLRDGAYVATCATAEVDQARLVEMMVGRRIE 240 Query: 246 EFYQHQRIAPADAAQLPTVMQVRALAGGKIRPAS-FDVRAGEVLGFAGLVGAGRTELARL 304 + + A + V++V AL + P S F +R GE+LGFAGLVG+GRTE Sbjct: 241 NCFP-PKPAKGGEGEGEVVLEVHALQLRRQAPVSQFQLRRGEILGFAGLVGSGRTETVLA 299 Query: 305 LFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATMNVAS 364 + GA + + G P+ P A++AGI +PE RK QGL ++ N ++N Sbjct: 300 MLGAHAALSCKLSMHGVPLRFADPAQALQAGIGLLPESRKEQGLITSFSILHNVSLNNYG 359 Query: 365 RHTRLGLVRSRSLGGVA-RAAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVL 423 ++ GL R A AA+QR+ VK + V LSGGNQQKV++ARW+ A KVL Sbjct: 360 KYRLGGLFLDRRREQQATEAAMQRVRVKAPGAQVRVDTLSGGNQQKVVIARWINHAMKVL 419 Query: 424 ILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGEL 483 I DEPTRG+D+ AKSEIYQL+ +QG ++++ISSELPEV+G+ DRV V R G I L Sbjct: 420 IFDEPTRGIDVGAKSEIYQLMREFTAQGYSILMISSELPEVVGMADRVCVFRGGGIVATL 479 Query: 484 AGAAITQENIMRLATDTNVPRTA 506 G A+ E IM AT + A Sbjct: 480 EGEAVNAEEIMTHATTGRIRHAA 502 Score = 105 bits (261), Expect = 5e-27 Identities = 67/233 (28%), Positives = 115/233 (49%), Gaps = 5/233 (2%) Query: 275 IRPASFDVRAGEVLGFAGLVGAGRTELARLLFGADPRSGGDILLEGRPVHIDQPRAAMRA 334 +R +VRAGEV G GAG++ L ++L G G+I ++G+P D RAA+ Sbjct: 17 LRGVDLEVRAGEVHALLGENGAGKSTLMKILCGIVRPDAGEIRIDGQPCRFDSYRAAIAG 76 Query: 335 GIAYVPEDRKGQGLFLQMAVAANATMNVASRHTRLGLVRSRSLGGVARAAIQRLNVKVAH 394 G+ V ++ + A + +R G ++ ++ A+ I +L V + Sbjct: 77 GVGVVFQEFS----LIPYLDAVDNMFLARELRSRWGWLQRAAMRRRAQEIIGQLGVAIPL 132 Query: 395 PETPVGKLSGGNQQKVLLARWLEIAPKVLILDEPTRGVDIYAKSEIYQLVHRLASQGVAV 454 + PV KLS QQ V +A+ L + ++L+LDEPT + ++ ++ L +QGVA+ Sbjct: 133 -DVPVCKLSVAQQQFVEIAKALALDARILVLDEPTATLTPAEVEHLFAVMRSLRAQGVAI 191 Query: 455 VVISSELPEVIGICDRVLVMREGMITGELAGAAITQENIMRLATDTNVPRTAP 507 + IS L E+ ICDR+ V+R+G A A + Q ++ + + P Sbjct: 192 IFISHHLEEIFEICDRITVLRDGAYVATCATAEVDQARLVEMMVGRRIENCFP 244 Lambda K H 0.320 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 723 Number of extensions: 31 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 521 Length of database: 502 Length adjustment: 35 Effective length of query: 486 Effective length of database: 467 Effective search space: 226962 Effective search space used: 226962 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory