Alignments for a candidate for iolE in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolE in Herbaspirillum seropedicae SmR1

Align Myo-inosose-2 dehydratase (EC 4.2.1.44) (characterized)
to candidate HSERO_RS12135 HSERO_RS12135 xylose isomerase

Query= reanno::HerbieS:HSERO_RS12135
         (306 letters)



>FitnessBrowser__HerbieS:HSERO_RS12135
          Length = 306

 Score =  621 bits (1601), Expect = 0.0
 Identities = 306/306 (100%), Positives = 306/306 (100%)

Query: 1   VATPFNVKIGINPISWMNDDLPSLGGETPLEQALSEGAEIGYRGFELGNKFPRQPEALRA 60
           VATPFNVKIGINPISWMNDDLPSLGGETPLEQALSEGAEIGYRGFELGNKFPRQPEALRA
Sbjct: 1   VATPFNVKIGINPISWMNDDLPSLGGETPLEQALSEGAEIGYRGFELGNKFPRQPEALRA 60

Query: 61  VLGKYQLECVSGWYSGQLATRSVEEEIVAVEAHLNLLAANGAQVMVYGEVADSIQGAPTP 120
           VLGKYQLECVSGWYSGQLATRSVEEEIVAVEAHLNLLAANGAQVMVYGEVADSIQGAPTP
Sbjct: 61  VLGKYQLECVSGWYSGQLATRSVEEEIVAVEAHLNLLAANGAQVMVYGEVADSIQGAPTP 120

Query: 121 LYQRPRFGSEAAWQGYAQRLDQFARHLLQRGVRLAYHHHMGAYVETPEDLDKLMALTGEE 180
           LYQRPRFGSEAAWQGYAQRLDQFARHLLQRGVRLAYHHHMGAYVETPEDLDKLMALTGEE
Sbjct: 121 LYQRPRFGSEAAWQGYAQRLDQFARHLLQRGVRLAYHHHMGAYVETPEDLDKLMALTGEE 180

Query: 181 VGLLFDSGHIAFAGGDPLAVLQRHLDRVCHVHCKDVRPAVVKLARNRDWSFLQAVINGAF 240
           VGLLFDSGHIAFAGGDPLAVLQRHLDRVCHVHCKDVRPAVVKLARNRDWSFLQAVINGAF
Sbjct: 181 VGLLFDSGHIAFAGGDPLAVLQRHLDRVCHVHCKDVRPAVVKLARNRDWSFLQAVINGAF 240

Query: 241 TVPGDGAIDFAPLLQLLYRRGYSGWLVVEAEQDPAVAPSYRYAQMGYRHLSTLVAAIENG 300
           TVPGDGAIDFAPLLQLLYRRGYSGWLVVEAEQDPAVAPSYRYAQMGYRHLSTLVAAIENG
Sbjct: 241 TVPGDGAIDFAPLLQLLYRRGYSGWLVVEAEQDPAVAPSYRYAQMGYRHLSTLVAAIENG 300

Query: 301 SLQEAA 306
           SLQEAA
Sbjct: 301 SLQEAA 306


Lambda     K      H
   0.320    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 522
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 306
Length of database: 306
Length adjustment: 27
Effective length of query: 279
Effective length of database: 279
Effective search space:    77841
Effective search space used:    77841
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate HSERO_RS12135 HSERO_RS12135 (xylose isomerase)
to HMM TIGR04379 (iolE: myo-inosose-2 dehydratase (EC 4.2.1.44))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR04379.hmm
# target sequence database:        /tmp/gapView.20208.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR04379  [M=290]
Accession:   TIGR04379
Description: myo_inos_iolE: myo-inosose-2 dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
   2.3e-130  420.3   0.0   2.7e-130  420.1   0.0    1.0  1  lcl|FitnessBrowser__HerbieS:HSERO_RS12135  HSERO_RS12135 xylose isomerase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__HerbieS:HSERO_RS12135  HSERO_RS12135 xylose isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  420.1   0.0  2.7e-130  2.7e-130       1     289 [.       6     293 ..       6     294 .. 0.99

  Alignments for each domain:
  == domain 1  score: 420.1 bits;  conditional E-value: 2.7e-130
                                  TIGR04379   1 kvklgiaPiaWvndDlpelggdttleqvlseaaeagfsgtElgnkfpkdpavLkaaleerglelvsgw 68 
                                                +vk+gi+Pi+W+ndDlp+lgg+t+leq+lse ae+g++g+Elgnkfp++p++L+a+l +++le+vsgw
  lcl|FitnessBrowser__HerbieS:HSERO_RS12135   6 NVKIGINPISWMNDDLPSLGGETPLEQALSEGAEIGYRGFELGNKFPRQPEALRAVLGKYQLECVSGW 73 
                                                59****************************************************************** PP

                                  TIGR04379  69 fsallleksveeeieavrehlellkalgakvivvaEvgksiqgdkdtplaerpkl.teeeweelaekl 135
                                                +s++l+++sveeei av++hl+ll+a ga+v+v++Ev++siqg   tpl +rp++ +e+ w+ +a++l
  lcl|FitnessBrowser__HerbieS:HSERO_RS12135  74 YSGQLATRSVEEEIVAVEAHLNLLAANGAQVMVYGEVADSIQGA-PTPLYQRPRFgSEAAWQGYAQRL 140
                                                *******************************************9.99********77889******** PP

                                  TIGR04379 136 nklgeilkekglklayHhHlgtvveteeeidrlmeltdpelvgllyDtGHlvfagedplavlekyadR 203
                                                ++++++l ++g++layHhH+g++vet e++d+lm+lt++e vgll+D+GH++fag+dplavl+++ dR
  lcl|FitnessBrowser__HerbieS:HSERO_RS12135 141 DQFARHLLQRGVRLAYHHHMGAYVETPEDLDKLMALTGEE-VGLLFDSGHIAFAGGDPLAVLQRHLDR 207
                                                **************************************99.*************************** PP

                                  TIGR04379 204 iahvHlKDvRkevleevrkekksFldavlkGvftvPGdGcidfeeilealkakdYeGWlvvEaEqDPa 271
                                                ++hvH+KDvR++v++ +r++++sFl+av++G+ftvPGdG+idf+++l+ l +++Y+GWlvvEaEqDPa
  lcl|FitnessBrowser__HerbieS:HSERO_RS12135 208 VCHVHCKDVRPAVVKLARNRDWSFLQAVINGAFTVPGDGAIDFAPLLQLLYRRGYSGWLVVEAEQDPA 275
                                                ******************************************************************** PP

                                  TIGR04379 272 kaepleyakkakkyleel 289
                                                +a++++ya++++++l++l
  lcl|FitnessBrowser__HerbieS:HSERO_RS12135 276 VAPSYRYAQMGYRHLSTL 293
                                                ***************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (290 nodes)
Target sequences:                          1  (306 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.76
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources