Alignments for a candidate for boxD in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for boxD in Herbaspirillum seropedicae SmR1

Align 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase (NADP+) (EC 1.2.1.77) (characterized)
to candidate HSERO_RS20590 HSERO_RS20590 enoyl-CoA hydratase

Query= BRENDA::Q13WK4
         (531 letters)



>FitnessBrowser__HerbieS:HSERO_RS20590
          Length = 686

 Score =  393 bits (1010), Expect = e-113
 Identities = 231/521 (44%), Positives = 310/521 (59%), Gaps = 12/521 (2%)

Query: 4   LLKNHVAGQWIAGTGAGITLTDPVTGVALVRVSSEGLDLARAFSFAREDGGAALRALTYA 63
           LL++ +AG+W+ G      L   V G A+     E +D   A   AR+ G  AL AL + 
Sbjct: 7   LLQSFIAGRWL-GQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALMALDFQ 65

Query: 64  QRAARLADIVKLLQAKRGDYYAIATANSGTTRNDSAVDIDGGIFTLSYYAKLGAS-LGEV 122
           QRAA L  + K L   +   YA++ A++G TR DS +DI+GG  TL  YA +GA+     
Sbjct: 66  QRAAILKALAKYLMEHKEVLYAVS-AHTGATRADSWIDIEGGSGTLFTYASIGANEFPSS 124

Query: 123 HALRDGSAESLSKDRSFSAQHVLSPTRGVALFINAFNFPSWGLWEKAAPALLSGVPVIVK 182
           + + +G A  L K   F+  H+L P  GVA+ INAFNFP WG+ EK AP  L+G+P IVK
Sbjct: 125 NLVHEGPAMRLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVK 184

Query: 183 PATATAWLTQRMVADVVDAGILPPGALSIICGSSAGLLDQIRSFDVVSFTGSADTAATLR 242
           PATAT++L +  V  +  +G+LP G+L ++ GS+  LL+++++ DVV+FTGSADTAA LR
Sbjct: 185 PATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTAAKLR 244

Query: 243 AHPAFVQRGARLNVEADSLNSAILCADATPDTPAFDLFIKEVVREMTVKSGQKCTAIRRA 302
           AHP  +      N EADSLN AIL  D T D   FDLF+KEV REMT K+GQKCTAIRRA
Sbjct: 245 AHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTAIRRA 304

Query: 303 FVPEAALEPVLEALKAKLAKITVGNPRNDAVRMGSLVSREQYENVLAGIAALREEAVLAY 362
            VP   ++ V E L A+L+KI VG+P  + VRMG L SR Q  +V   +A LR  A L  
Sbjct: 305 IVPRRHVDAVAERLAARLSKIVVGDPALETVRMGPLASRAQQRDVEERVALLRHSADLIL 364

Query: 363 DS-SAVPLIDADANIAACVAPHLFVVNDPDNATLLHDVEVFGPVASVAPYRVTTDTNALP 421
            + SA           A  AP L +  D    + +H+VE FGPV+++ PY      + L 
Sbjct: 365 GAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPY------DDLD 418

Query: 422 EAHAVALARRGQGSLVASIYSNDDAHLGRLALELADSHGRVHAISPSVQHSQTGHGNVMP 481
           E  A+ALA RG+GSLVAS+ +   A   R     A  HGR+  +        TGHG+ +P
Sbjct: 419 E--ALALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLP 476

Query: 482 MSLHGGPGRAGGGEELGGLRALAFYHRRSAIQAASAAIGTL 522
              HGGPGRAGGGEELGG RA+  Y +R+A+Q +   +  +
Sbjct: 477 QLKHGGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAV 517


Lambda     K      H
   0.318    0.132    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 770
Number of extensions: 33
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 531
Length of database: 686
Length adjustment: 37
Effective length of query: 494
Effective length of database: 649
Effective search space:   320606
Effective search space used:   320606
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources