Aligments for a candidate for pad-dh in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Aligments for a candidate for pad-dh in Herbaspirillum seropedicae SmR1

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate HSERO_RS05115 HSERO_RS05115 aldehyde dehydrogenase

Query= BRENDA::P05091
         (517 letters)



>FitnessBrowser__HerbieS:HSERO_RS05115
          Length = 793

 Score =  353 bits (906), Expect = e-101
 Identities = 197/446 (44%), Positives = 268/446 (60%), Gaps = 15/446 (3%)

Query: 57  VNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDR 116
           ++P++G+++  +++G   DVD AV+AA+AA      W+ +    R R L  LA  ++R  
Sbjct: 51  IDPASGKLLAHLSQGSAADVDAAVQAAQAALP---GWQALGGHGRARHLYALARTVQRHA 107

Query: 117 TYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPV 176
             LA LETLDNGKP   S  VD+ +V +   +YAGWA     +          +  H PV
Sbjct: 108 RLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQLQESE----------FPDHVPV 157

Query: 177 GVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVN 236
           GV GQI+PWNFPLLM AWK+ PALA GN VV+K AE T LTAL  A L ++AG P GV+N
Sbjct: 158 GVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTALLFAELAQQAGLPAGVLN 217

Query: 237 IVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMS 296
           IV G G T GAA+ +H  + K+AFTGSTE+GR+I+     S  K +TLELGGKSP I+  
Sbjct: 218 IVTGDGAT-GAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSG-KSLTLELGGKSPFIVFE 275

Query: 297 DADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKT 356
           DAD+D A+E    A++FNQGQ CCAGSR  VQE I+D F+ R   R +   VG P D  +
Sbjct: 276 DADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPLDKCS 335

Query: 357 EQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEE 416
           + G  +   Q +++   +  G +EGA+           G F  PT+   V    T+A EE
Sbjct: 336 DMGALISPVQLERVRSLVEQGVREGAQCHQVVLDTPPGGCFYPPTLLTGVHPAATVASEE 395

Query: 417 IFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVF 476
           IFGPV+  + F+T +E V  ANNS YGLAA+++++ +  A  ++  L+AG VW+N  + F
Sbjct: 396 IFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGVAPQLKAGVVWINSTNQF 455

Query: 477 GAQSPFGGYKMSGSGRELGEYGLQAY 502
            A   FGG + SG GRE G  G   Y
Sbjct: 456 DAAVGFGGVRESGYGREGGREGCYEY 481



 Score = 75.9 bits (185), Expect = 5e-18
 Identities = 62/226 (27%), Positives = 96/226 (42%), Gaps = 6/226 (2%)

Query: 62  GEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDRTYLAA 121
           GE +  V  G+++D+  AV AA  A      W       R + L  +A+ +   RT   A
Sbjct: 542 GERLEDVGLGNRKDIRNAVAAAVKA----GGWTSASPHRRAQGLYYIAENLSA-RTEEFA 596

Query: 122 LETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQ 181
                           ++D  +K L  Y  WADK+ G          +    E +GV G 
Sbjct: 597 RRIASTTGVTADEARAEVDASIKRLFTYGAWADKFEGAVHQPPMRGVALAMPEAIGVVGV 656

Query: 182 IIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGF 241
           + P   PLL     + P +A GN VV+  +EQ PL A  +  ++  +  P GV+NIV G 
Sbjct: 657 VCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLVATDLYQVLDTSDLPAGVINIVTGK 716

Query: 242 GPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELG 287
                  +A H++V+ +   G  E     +    + NLKR  ++ G
Sbjct: 717 SAELLPVLAEHDEVEALWVRGPAEFSATAE-RLSTGNLKRCFVDHG 761


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 952
Number of extensions: 51
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 517
Length of database: 793
Length adjustment: 38
Effective length of query: 479
Effective length of database: 755
Effective search space:   361645
Effective search space used:   361645
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources