Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate HSERO_RS14710 HSERO_RS14710 amino acid ABC transporter ATPase
Query= uniprot:A0A165KC78 (242 letters) >FitnessBrowser__HerbieS:HSERO_RS14710 Length = 243 Score = 238 bits (608), Expect = 6e-68 Identities = 117/240 (48%), Positives = 170/240 (70%) Query: 2 AEKSNKVLLQVKGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGKTTTMKAITGTLSM 61 A + + +LQV+ L VAYG I+AVKG+ + EGE+ +L+G+NGAGK+T++ AI+G + Sbjct: 3 ASGNKQPILQVEDLAVAYGHIEAVKGISLSLNEGEITALVGANGAGKSTSLLAISGLVKA 62 Query: 62 NDGNIEYLGKSIKGKGAWDLVKEGLVMVPEGRGVFARMTITENLQMGAYIRKDKAGILAD 121 G + G+ + +V+ G+V V EGR +T+ ENL +GAY RKDK G+ D Sbjct: 63 QRGRVLLHGQDLLQMSPHRIVESGVVQVAEGRATLTTLTVEENLGLGAYTRKDKDGVARD 122 Query: 122 IEKMFTIFPRLRERKDQLAGTMSGGEQQMLAMGRALMSQPKVLLLDEPSMGLSPIMVDKI 181 +E ++++FP L+ R LAG +SGGEQQMLA+GRALM++PKVLLLDEPSMGL+P+++ +I Sbjct: 123 LEWVYSLFPVLKNRAAGLAGNLSGGEQQMLAIGRALMAKPKVLLLDEPSMGLAPLIIQEI 182 Query: 182 FEVVRDVYALGVTIVLVEQNASRALAIADRGYVMESGLITMTGPGQQLLNDPKVRAAYLG 241 F +V+++ G+T++LVEQN +AL IA RGYV+E+G I + G LL +PKV AYLG Sbjct: 183 FRIVQEINKTGMTVLLVEQNVRQALRIAQRGYVLETGKIVLEDSGANLLGNPKVVEAYLG 242 Lambda K H 0.317 0.136 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 184 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 243 Length adjustment: 23 Effective length of query: 219 Effective length of database: 220 Effective search space: 48180 Effective search space used: 48180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory