Align HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized)
to candidate HSERO_RS08240 HSERO_RS08240 sulfate ABC transporter ATP-binding protein
Query= TCDB::Q9KKE1 (275 letters) >FitnessBrowser__HerbieS:HSERO_RS08240 Length = 359 Score = 168 bits (426), Expect = 1e-46 Identities = 94/236 (39%), Positives = 142/236 (60%), Gaps = 10/236 (4%) Query: 33 ILSRSGCTVGLNDVSLKIGAGKIFVIMGLSGSGKSTLVRHINRLIEPTSGEVLFDGDNIL 92 I R G LNDVSL AG++ ++G SG GK+TL+R I L P SG+VL DG++ Sbjct: 8 IQKRFGSFTALNDVSLNFPAGELTALLGPSGCGKTTLLRIIAGLESPDSGQVLLDGEDA- 66 Query: 93 DLGAKALRAFRMRRVSMVFQSFALMPHRTVLQNVVYGQRVRGVSKDDA----REIGMKWI 148 + R R R+V VFQ +AL H TV +N+ +G RV+ S+ + RE + + Sbjct: 67 -----SARHVRERQVGFVFQHYALFKHMTVFENIAFGLRVKPRSQRPSEAVIREKVKRLL 121 Query: 149 DTVGLSGYDAKFPHQLSGGMKQRVGLARALAADTDVILMDEAFSALDPLIRGDMQDQLLQ 208 + V L ++P QLSGG +QR+ LARALA + V+L+DE F ALD +R +++ L + Sbjct: 122 ELVQLDWLADRYPPQLSGGQRQRIALARALAVEPRVLLLDEPFGALDAKVRKELRRWLRR 181 Query: 209 LQRNLAKTIVFITHDLDEALRIGSEIAILRDGQVVQVGTPNDILDNPANDYVARFV 264 L L T +F+THD +EAL + ++ ++ GQV Q+GTP + ++PA+ +V F+ Sbjct: 182 LHDELHVTSIFVTHDQEEALEVADQVVLMNKGQVEQIGTPEAVYNHPASPFVYGFL 237 Lambda K H 0.323 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 253 Number of extensions: 20 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 359 Length adjustment: 27 Effective length of query: 248 Effective length of database: 332 Effective search space: 82336 Effective search space used: 82336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory