Alignments for a candidate for actP in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Herbaspirillum seropedicae SmR1

Align Sodium: solute symporter family protein (characterized, see rationale)
to candidate HSERO_RS03215 HSERO_RS03215 sodium:solute symporter

Query= uniprot:E4PHQ0
         (588 letters)



>FitnessBrowser__HerbieS:HSERO_RS03215
          Length = 683

 Score =  209 bits (531), Expect = 4e-58
 Identities = 127/351 (36%), Positives = 192/351 (54%), Gaps = 38/351 (10%)

Query: 233 GFNEYTADIDNKLNMVLFTLSLMIGTAGLPHVIIRFFTVPKVADARWSAGWALVFIALLY 292
           G ++ ++DI    N +     LM+GTA LPH+++R++T P VA AR S  W L FI L+Y
Sbjct: 367 GRDQESSDIKRN-NFLALVFCLMLGTAALPHILMRYYTTPTVAGARTSVFWTLFFIMLIY 425

Query: 293 LTAPAVASMARLNLMTTIYPDGTSAEPIQYDERPNWIKEW----EITGLIQFTDKNEDGR 348
           LT PA+  + + ++ T++   G+S     Y   P+W+  W    ++  L+  TD N DG 
Sbjct: 426 LTVPALTVLVKYDIYTSLV--GSS-----YSHLPDWVYYWANMDKLNPLVSITDLNRDGL 478

Query: 349 IQLYNDSEAFAPTAEARGWNGNELVVNRDILVLANPEIANLPGWVIGLIAAGGLAAALST 408
           +QL                   E+ ++ DI+VLA PEIA LP +V GL+AAGGLAAALST
Sbjct: 479 VQL------------------GEISMDGDIIVLAMPEIAGLPYFVSGLVAAGGLAAALST 520

Query: 409 AAGLLLAISSAVSHDLIKGSINPAISEKGELLAARISMAVAIVVATYLGANPPGFAAQVV 468
           A GLLL IS+A+SHD+    ++P+ S +  +  +++ + V  ++A Y  +  PG    +V
Sbjct: 521 ADGLLLTISNALSHDVYYKVVDPSASTQKRVTISKLLLLVVALLAAYAASLKPGDILSMV 580

Query: 469 ALAFGIAAASLFPALMMGIFSKRVNNTGAIAGMLSGLTFTLVYIFVYKGWLFIPGTNNLP 528
             AF +AA++LFPAL++G+F KR N  GAIAG+L G    + Y+        +    +  
Sbjct: 581 GAAFSLAASTLFPALVLGVFWKRANQAGAIAGILCGFLMCVYYM--------LHTNPSFG 632

Query: 529 DTPDNWVLGISPLSIGAIGAIVNFAVAFIVSNATEEPPVEIQELVESVRYP 579
            +       I+P+S G  G  V  A   IVS  T  P      L++ +R P
Sbjct: 633 GSAAGQWFHIAPISAGIFGVPVGMAAMVIVSLLTPPPDEATIALIDHIRTP 683



 Score =  175 bits (443), Expect = 6e-48
 Identities = 87/233 (37%), Positives = 143/233 (61%), Gaps = 12/233 (5%)

Query: 6   INIIFVGGSFLLYIAIAIWAKAGSTSDFYVAGGGVHPITNGAAIGADWMSAASFISMAGL 65
           I  +F+  +  LY  I +  +     ++YVAG  V P+ NG A  ADW+SAA+FIS+AG 
Sbjct: 42  IGYLFLFVTIGLYAGIGVVCRTSDVPEYYVAGRRVPPLFNGMATAADWISAATFISLAGG 101

Query: 66  IAAGGYANSTFLMGWTGGYVLLAMLLAPYLRKFGKFTVPEFIGDRFYSKNA-------RL 118
           +   G+    +++GWTGGY L+A+L+APYLRKFG++T+ +F+  R+  +         R+
Sbjct: 102 LYLQGFDGLAYILGWTGGYCLVALLIAPYLRKFGQYTIADFLAARYPGRPGSQGVNVIRI 161

Query: 119 VAVICLIVASVTYVIGQMAGAGVAFSRFLEVDSTVGLMIAAVVVFVYAVMGGMKGITYTQ 178
           +AV   I+ S TYV+ Q+ G G+  SRF  +D ++G+ +    + V + +GGM+ IT+TQ
Sbjct: 162 LAVAATILISFTYVVAQIYGVGLITSRFTGIDFSIGIFLGLASILVCSFLGGMRAITWTQ 221

Query: 179 VAQYCVLIVAYTIPAVFISLQLTGNPIPGLGLFSTHVDSGMPILSKLNQVITD 231
           VAQY ++++AY IP +++S + T  P+P +   +      +P LS + Q + D
Sbjct: 222 VAQYIIILLAYLIPVIWLSAKHTTVPVPPVAYGAV-----LPKLSAIEQRLED 269


Lambda     K      H
   0.323    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 988
Number of extensions: 44
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 588
Length of database: 683
Length adjustment: 38
Effective length of query: 550
Effective length of database: 645
Effective search space:   354750
Effective search space used:   354750
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources