Alignments for a candidate for cstA in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for cstA in Herbaspirillum seropedicae SmR1

Align Carbon starvation protein A (characterized, see rationale)
to candidate HSERO_RS09795 HSERO_RS09795 carbon starvation protein A

Query= uniprot:A0A0C4Y7X7
         (690 letters)



>FitnessBrowser__HerbieS:HSERO_RS09795
          Length = 689

 Score = 1072 bits (2772), Expect = 0.0
 Identities = 526/689 (76%), Positives = 611/689 (88%), Gaps = 1/689 (0%)

Query: 1   MNRIGQHLVWLAVAILGAFAFATVALSRGEAVSALWIVVAAICIYLIAYRYYSKFIAEKV 60
           MNRI   L WLA+A+ GA A A VAL RGE+++A+W+VVAA+C+YLIAYR+YSKFIA++V
Sbjct: 1   MNRIVSRLGWLALALAGAGACAYVALQRGESINAVWLVVAAVCVYLIAYRFYSKFIADRV 60

Query: 61  MQLDPKRMTPAWRHNDGLDYVPTNKAVLFGHHFAAIAGAGPLVGPVLAAQMGYMPGMLWI 120
           + LD  RMTPA++ NDGLDYVPTNK VLFGHHFAAIAGAGPLVGPVLAAQMGY+PGMLWI
Sbjct: 61  LGLDGGRMTPAYKFNDGLDYVPTNKYVLFGHHFAAIAGAGPLVGPVLAAQMGYLPGMLWI 120

Query: 121 LAGVVFAGAVQDFMVLFISTRRDGRSLGDLIKSEMGTVPGMIALFGCFMIMIIILAVLAL 180
           LAGVVFAGAVQDFMVLFISTRRDGRSLGDLIKSE+G VPG+IALFG FMIM+IILAVLAL
Sbjct: 121 LAGVVFAGAVQDFMVLFISTRRDGRSLGDLIKSELGQVPGVIALFGAFMIMVIILAVLAL 180

Query: 181 IVVKALAGSPWGTFTVGVTIPIALFMGIYTRYIRPGRIGEVSVIGFVLLMLAIIGGQYVH 240
           IVVKALA SPWG+FTV  TIPIALFMG+Y+RYIRPGRIGEVS IGFVLLMLAI+GGQYV 
Sbjct: 181 IVVKALAESPWGSFTVAATIPIALFMGVYSRYIRPGRIGEVSWIGFVLLMLAIVGGQYVQ 240

Query: 241 ESAVLAPLFTYDGKALTWMLIIYGFIAAVLPVWLLLAPRDYLSTFLKIGTIIALAIGILI 300
           E   L  +FT+DGK LTWMLI YGF+A+VLPVWLLLAPRDYLSTFLKIGTI+ALA+GI+ 
Sbjct: 241 EHPALGQMFTFDGKQLTWMLIGYGFVASVLPVWLLLAPRDYLSTFLKIGTILALALGIVF 300

Query: 301 VAPELKMPAFTQFAKGGGPVWSGNLFPFLFITIACGAVSGFHALISSGTTPKLLESEAHM 360
           +AP LKM A T+F  G GPVWSG+LFPFLFITIACGAVSGFHALISSGTTPKLLE+E H 
Sbjct: 301 IAPHLKMHAVTRFIDGSGPVWSGSLFPFLFITIACGAVSGFHALISSGTTPKLLENEKHA 360

Query: 361 RFIGYGAMLAESFVAIMALVAASVIEPGVYFAMNSPAAVIGTSPESVAQVVSGWGFVITP 420
           RFIGYGAML ESFVAIMAL+AAS IEPG+YFAMNSPAA+IGT+PE+VAQV+S WGF ITP
Sbjct: 361 RFIGYGAMLMESFVAIMALIAASCIEPGIYFAMNSPAALIGTTPENVAQVISQWGFHITP 420

Query: 421 DVLIQTAKDVGENSIISRAGGAPTLAVGIAHILHQVVGGQAMMAFWYHFAILFEALFILT 480
           ++L  TA+ VGE++IISRAGGAPTLAVG+A IL  V  G+AMMAFWYHFAILFEALFILT
Sbjct: 421 EMLTHTAQAVGEHTIISRAGGAPTLAVGMAQILSGVT-GEAMMAFWYHFAILFEALFILT 479

Query: 481 AVDAGTRAGRFMLQDLLGSFVPSFRRTDSLPANLIATALTVSFWGYFLYQGVVDPLGGIN 540
           AVDAGTRAGRFMLQDLLG+F+P  +RTDS  A+LIAT L V+ WGYFLYQGVVDPLGGIN
Sbjct: 480 AVDAGTRAGRFMLQDLLGAFIPPMKRTDSWVASLIATGLCVAGWGYFLYQGVVDPLGGIN 539

Query: 541 TLWPLFGISNQMLAAVALVLGTCVLVKMKRGQYAWVTLVPTIWLLICTLTAGWQKLFDAD 600
           TLWPLFGISNQMLA +AL+L TCVL KMKR ++AWVT++PT WLL CTLTAGWQK+FDA+
Sbjct: 540 TLWPLFGISNQMLAGIALILATCVLFKMKRDRFAWVTVLPTAWLLACTLTAGWQKVFDAN 599

Query: 601 PKVSFLTHAAKFSDAIAQGKVLAPAKSMEQMHRIVFNDYLDAGLCALFMFVVLSIVFYGF 660
           P+V FL HA+K+  A+ +GK+LAPAKS+EQM +++FNDY+DAGL A+FM VV+S++ +G 
Sbjct: 600 PRVGFLAHASKYQAALDEGKLLAPAKSIEQMRQVIFNDYVDAGLAAMFMLVVISVLVFGI 659

Query: 661 KTAMKARAENRPTDRETPFEPMPASASAQ 689
           +T +KAR+ ++P+ +E P+E +PASA AQ
Sbjct: 660 RTVLKARSAHQPSVKEAPYETLPASALAQ 688


Lambda     K      H
   0.328    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1495
Number of extensions: 51
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 690
Length of database: 689
Length adjustment: 39
Effective length of query: 651
Effective length of database: 650
Effective search space:   423150
Effective search space used:   423150
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources