Alignments for a candidate for rbsA in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for rbsA in Herbaspirillum seropedicae SmR1

Align Ribose import ATP-binding protein RbsA 2, component of D-ribose porter (Nanavati et al., 2006). Induced by ribose (characterized)
to candidate HSERO_RS22220 HSERO_RS22220 D-ribose transporter ATP-binding protein

Query= TCDB::Q9X051
         (523 letters)



>FitnessBrowser__HerbieS:HSERO_RS22220
          Length = 505

 Score =  408 bits (1048), Expect = e-118
 Identities = 220/500 (44%), Positives = 325/500 (65%), Gaps = 10/500 (2%)

Query: 12  LLEARNITKTFPGVIAVNNVTLQIYKGEVCALVGENGAGKSTLMKILAGVYPDYEGQIFL 71
           +L    I K F GV+A+ +V   +  GEV AL+GENGAGKSTL+KIL G++   EG I L
Sbjct: 12  VLSLSGIGKRFQGVVALQDVGFTVRPGEVMALLGENGAGKSTLVKILTGIHQPDEGSIHL 71

Query: 72  EGKEVRFRNPREAQENGIALIPQELDLVPNLSSAENIFLSREPV-NEFGVIEYQKMFEQA 130
            G+EVRF + ++A   GI  + QE  +   LS AENI++ R+P+      I++++M ++A
Sbjct: 72  GGREVRFASAQDAMRGGITAVHQETVMFEELSVAENIWIGRQPLCGTPRRIDWRRMEDEA 131

Query: 131 SKLFSKLGVNIDPKTKVEDLSTSQQQMVAIAKALSLDAKIIIMDEPTSAIGKRETEQLFN 190
             LF++L V++  + +V+DLS +Q+  V IA+ALS  A+++IMDEPT+A+   E  +L+ 
Sbjct: 132 RALFARLEVDLPVRARVKDLSVAQRHFVEIARALSQQAQVVIMDEPTAALSHHEIGELYR 191

Query: 191 IIRSLKNEGKSVIYISHRLEEIFEIADRVVVMRDGRKVGEGPIEEFDHDKLVRLMVGRSI 250
           II  L+  G +VI+ISH+ +EI+ +ADR  V+RDGR +  G + +    +LV LMVGR +
Sbjct: 192 IIGQLRRAGTAVIFISHKFDEIYAVADRYTVLRDGRFIASGELADITEQQLVALMVGREV 251

Query: 251 DQFFIKERATITDEIFRVEGIKLWSLDRKKLLVDDVSFYVRKGEVLGIYGLVGAGRTELL 310
            Q F +  +   D+   V  +K  S   +    DDVSF VR GE+LG YGLVGAGR+E++
Sbjct: 252 GQVFSRAASNTEDQTAPVLEVKHLSHPSE---FDDVSFAVRPGEILGFYGLVGAGRSEVM 308

Query: 311 EAIFGAHPGRTEGKVFIGGKEIKIHSPRDAVKNGIGLVPEDRKTAGLILQMSVLHNITLP 370
            A+FG  P   +G V+I G+E+K+ SP  A+ +G+  VPEDR+  G +L + +  NITLP
Sbjct: 309 HALFGLSP-EAQGAVWIDGREVKLCSPAQAIAHGLAYVPEDRQRQGALLSLPIFQNITLP 367

Query: 371 SVV-MKLIVRKFGLIDSQLEKEIVRSFIEKLNIKTPSPYQIVENLSGGNQQKVVLAKWLA 429
            +  +   +R+      + E +I R   E+L +K    +Q V  LSGGNQQKVVLAKWLA
Sbjct: 368 VLPGIGFFLRRH----RRREIDIARRLCEQLELKASHFHQHVAQLSGGNQQKVVLAKWLA 423

Query: 430 IKPKVLLLDEPTRGIDVNAKSEIYKLISEMAVSGMGVVMVSSELPEILAMSDRILVMSEG 489
            +P+VL+LDEPT+GID+ +K+ +++ I E+   G+ V++VSSELPE++ MSDRI+VM +G
Sbjct: 424 TQPRVLILDEPTKGIDIGSKAAVHRFIGELVAQGLAVILVSSELPEVMGMSDRIVVMHQG 483

Query: 490 RKTAEFLREEVTEEDLLKAA 509
           R    F R E + E L  AA
Sbjct: 484 RVQQVFSRAEASAEALAAAA 503



 Score =  105 bits (263), Expect = 3e-27
 Identities = 67/232 (28%), Positives = 129/232 (55%), Gaps = 8/232 (3%)

Query: 283 VDDVSFYVRKGEVLGIYGLVGAGRTELLEAIFGAHPGRTEGKVFIGGKEIKIHSPRDAVK 342
           + DV F VR GEV+ + G  GAG++ L++ + G H    EG + +GG+E++  S +DA++
Sbjct: 28  LQDVGFTVRPGEVMALLGENGAGKSTLVKILTGIHQP-DEGSIHLGGREVRFASAQDAMR 86

Query: 343 NGIGLVPEDRKTAGLILQMSVLHNITLPSVVMKLIVRKFGLIDSQLEKEIVRSFIEKLNI 402
            GI  V ++     +  ++SV  NI +    +    R+   ID +  ++  R+   +L +
Sbjct: 87  GGITAVHQETV---MFEELSVAENIWIGRQPLCGTPRR---IDWRRMEDEARALFARLEV 140

Query: 403 KTPSPYQIVENLSGGNQQKVVLAKWLAIKPKVLLLDEPTRGIDVNAKSEIYKLISEMAVS 462
             P   + V++LS   +  V +A+ L+ + +V+++DEPT  +  +   E+Y++I ++  +
Sbjct: 141 DLPVRAR-VKDLSVAQRHFVEIARALSQQAQVVIMDEPTAALSHHEIGELYRIIGQLRRA 199

Query: 463 GMGVVMVSSELPEILAMSDRILVMSEGRKTAEFLREEVTEEDLLKAAIPRSV 514
           G  V+ +S +  EI A++DR  V+ +GR  A     ++TE+ L+   + R V
Sbjct: 200 GTAVIFISHKFDEIYAVADRYTVLRDGRFIASGELADITEQQLVALMVGREV 251


Lambda     K      H
   0.317    0.137    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 561
Number of extensions: 24
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 523
Length of database: 505
Length adjustment: 35
Effective length of query: 488
Effective length of database: 470
Effective search space:   229360
Effective search space used:   229360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources