Align D-gluconate dehydrogenase dehydrogenase subunit (EC 1.1.99.3) (characterized)
to candidate HSERO_RS22805 HSERO_RS22805 GMC family oxidoreductase
Query= metacyc::MONOMER-12745 (594 letters) >FitnessBrowser__HerbieS:HSERO_RS22805 Length = 590 Score = 573 bits (1478), Expect = e-168 Identities = 286/589 (48%), Positives = 392/589 (66%), Gaps = 9/589 (1%) Query: 8 VDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYP-DGNYPQVIDELTYSVRKKLF 66 VD VIVG GW G++MA EL +AGL V ALERGP D P D YP+ D+ Y+ R K+F Sbjct: 9 VDVVIVGLGWAGSLMAIELAQAGLKVRALERGP--DRPPEDFAYPKPADQYGYATRNKVF 66 Query: 67 QDISKETVTIRHSVNDVALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRMRSHYEE 126 +T+R++ ALP R+ GAF PG GVGG+GLHW+ V R P +L+++++ ++ Sbjct: 67 ATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKLKTYADQ 126 Query: 127 RYGKNFIPKDMTIQDFGVSYEELEPFFDYAEKVFGTSGQAWTVRGQLVGDGKGGNPYAPD 186 Y + + +M I+DF ++ E+EP +D+ +KV G SG +RGQ+ GG+P+ Sbjct: 127 AYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQ---PGGDPFEGP 183 Query: 187 RSDHFPLESQKNTYSAQLFQKAANDVGYKPYNLPSANTSGPYTNPYGAQMGPCNFCGFCS 246 RS+ +PL + +TY+ LF A +GY P+ PSAN S +TNPYGAQ+ PCN+CGFCS Sbjct: 184 RSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCGFCS 243 Query: 247 GYVCYMYSKASPNVNILPALKPLPNFELRPNSHVLRVNLDSSKTRATGVTYVDGQGREIE 306 Y C YSKASP IL +K LPNF+ + N++V+RV+L + K A GVTYVD +E+ Sbjct: 244 KYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMKEVF 303 Query: 307 QPADLVILGAFQFHNVRLMLLSGIGKPYDPITGEGVVGKNFAYQNMATIKAYFDKDVHTN 366 QPA +VIL +FQF NVRLMLLSGIGKPYDPIT GV+G+N+A+ + +F KD H N Sbjct: 304 QPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFF-KDKHFN 362 Query: 367 NFIGAGGNGVAVDDFNADNFDHGPH-GFVGGSPMWVNQAGSRPIAGTSNPPGTPAWGSAW 425 +F AG G +D + NFD G GF+GG+ + +QA PI GT+ PPGTP+WG W Sbjct: 363 SFATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPI-GTALPPGTPSWGRGW 421 Query: 426 KKATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGLPLLRMTFDWQENDIKMNRFM 485 K+ D+Y H + + SYRG++LDLDP Y+D +G PLLRMTFDW++N++K+ R++ Sbjct: 422 KEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRYL 481 Query: 486 VEKMGKIAEAMNPKAIALLGKKVGEHFNTASYQTTHLNGGAIMGTDPKTSALNRYLQSWD 545 + + KI++ +NP ++ + H++ Y +TH GGAIMG P+ SALN+YLQSWD Sbjct: 482 RDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSWD 541 Query: 546 VHNVFVPGASAFPQGLGYNPTGLVAALTYWSARAIREQYLKNPGPLVQA 594 VHNVFVPG +AFPQ NPT + A+T +A AI+ QYLKNPGPLVQA Sbjct: 542 VHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVQA 590 Lambda K H 0.318 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1276 Number of extensions: 70 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 594 Length of database: 590 Length adjustment: 37 Effective length of query: 557 Effective length of database: 553 Effective search space: 308021 Effective search space used: 308021 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory