GapMind for catabolism of small carbon sources

 

Alignments for a candidate for thuG in Herbaspirillum seropedicae SmR1

Align Maltose transport system permease protein malG aka TT_C1629, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate HSERO_RS17010 HSERO_RS17010 sugar ABC transporter permease

Query= TCDB::Q72H66
         (280 letters)



>FitnessBrowser__HerbieS:HSERO_RS17010
          Length = 270

 Score =  154 bits (390), Expect = 2e-42
 Identities = 89/262 (33%), Positives = 140/262 (53%), Gaps = 3/262 (1%)

Query: 6   RLLGRLFFYLLVVFVVVYSVFPFYWAVISSFKPSDALFSPDPSFLPVPFTLEHYENVFLQ 65
           RL+ R   + + + +V+ +VFP  WA+++S K    + +P P FL  P TLE+Y  V   
Sbjct: 3   RLITRCAVWGVGIVLVLVAVFPLLWALLNSVKTLLDIVTPTPRFLFTP-TLENYRQVIGS 61

Query: 66  ANFGRNLLNSLIVAGGATLLSLVLGVLAAYALGRLPFPPKNAVMYIVLSMTMFPQIAVLG 125
                 L NS ++ G A LL   +GV AAY + R   P K  + + +LS+   P +AV  
Sbjct: 62  PEVLVGLTNSAVIVGSAVLLGTFMGVPAAYVIARYHVPGKRDIQFFLLSLRFLPPVAVAI 121

Query: 126 GLFLLLRQTGLFNTHLGLILTYLLFTLPFTVWVLVGYFRGLPRELEEAAYVDGATPLQTL 185
            L  +    GL++T   +I+TYLL TL    W+ +  F+ +PRE+EEAA +DG  P    
Sbjct: 122 PLIAIWVDLGLYDTRFSMIVTYLLTTLSTITWLSIPVFQRMPREIEEAATLDGYGPYAVF 181

Query: 186 LKVMLPLTGPGLVTTGLLAFIAAWNEYLFALTFTVGDSVKTVPPAIASFGGATPFEIPWG 245
            K+ LP     L+   + +F+  WNE + AL  T  +S     P +AS   +   E+PWG
Sbjct: 182 WKIALPNCATTLLGGIIFSFVLVWNELMIALALTSSNSATL--PVVASAFTSMGQEVPWG 239

Query: 246 SIMAASVVVTVPLVVLVLVFQQ 267
            I A++V++ +P ++ V V  +
Sbjct: 240 VINASTVLLALPPLIFVGVLSR 261


Lambda     K      H
   0.329    0.145    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 153
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 280
Length of database: 270
Length adjustment: 25
Effective length of query: 255
Effective length of database: 245
Effective search space:    62475
Effective search space used:    62475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory