Align β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized)
to candidate HSERO_RS19990 HSERO_RS19990 acetyl-CoA acetyltransferase
Query= metacyc::MONOMER-15952 (401 letters) >FitnessBrowser__HerbieS:HSERO_RS19990 Length = 400 Score = 577 bits (1486), Expect = e-169 Identities = 289/401 (72%), Positives = 338/401 (84%), Gaps = 1/401 (0%) Query: 1 MNEALIIDAVRTPIGRYAGALASVRADDLGAIPLKALIARHPQLDWSAVDDVIYGCANQA 60 M + I DA+RTPIGRY GAL VRADDLGA+PL+AL+ R+PQ+DW AVDDVIYGCANQA Sbjct: 1 MKDVFICDAIRTPIGRYGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQA 60 Query: 61 GEDNRNVARMAALLAGLPVSVPGTTLNRLCGSGLDAVGSAARALRCGEAGLMLAGGVESM 120 GEDNRNVARM+ LLAGLP VPG+T+NRLCGSG+DA+G+AARA++ GE LM+AGGVESM Sbjct: 61 GEDNRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVESM 120 Query: 121 SRAPFVMGKSEQAFGRSAEIFDTTIGWRFVNKLMQQGFGIDSMPETAENVAAQFNISRAD 180 +RAPFVMGK++ AF R A I DTTIGWRFVN LM+Q +G+D+MPETAENVA F +SRAD Sbjct: 121 TRAPFVMGKADSAFSRQAAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRAD 180 Query: 181 QDAFALRSQHKAAAAIANGRLAKEIVAVEIAQRKGPAKIVEHDEHPRGDTTLEQLAKLGT 240 QD FA+RSQ KAAAA ANG LA+EIV V I Q+KG V DEHPR T++E LA+L Sbjct: 181 QDQFAVRSQAKAAAAQANGTLAQEIVPVVIPQKKGDPITVTQDEHPRA-TSMEALARLKG 239 Query: 241 PFRQGGSVTAGNASGVNDGACALLLASSEAAQRHGLKARARVVGMATAGVEPRIMGIGPV 300 + GSVTAGNASGVNDGACALLLAS+EA +++ LK RAR++GMATAGV PRIMG+GP Sbjct: 240 VVKPDGSVTAGNASGVNDGACALLLASAEAVEKYQLKPRARILGMATAGVAPRIMGMGPA 299 Query: 301 PATRKVLELTGLALADMDVIELNEAFAAQGLAVLRELGLADDDERVNPNGGAIALGHPLG 360 PA++KVL G+ + MDVIELNEAFA+QGLAVLRELG+ADDD RVNPNGGAIALGHPLG Sbjct: 300 PASKKVLAQLGMTIDQMDVIELNEAFASQGLAVLRELGVADDDARVNPNGGAIALGHPLG 359 Query: 361 MSGARLVTTALHELEERQGRYALCTMCIGVGQGIALIIERI 401 MSGARLVTTA ++LE GRYALCTMCIGVGQGIA++IER+ Sbjct: 360 MSGARLVTTATYQLERTGGRYALCTMCIGVGQGIAMVIERV 400 Lambda K H 0.319 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 549 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 401 Length of database: 400 Length adjustment: 31 Effective length of query: 370 Effective length of database: 369 Effective search space: 136530 Effective search space used: 136530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate HSERO_RS19990 HSERO_RS19990 (acetyl-CoA acetyltransferase)
to HMM TIGR02430 (pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02430.hmm # target sequence database: /tmp/gapView.6628.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02430 [M=400] Accession: TIGR02430 Description: pcaF: 3-oxoadipyl-CoA thiolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-227 740.6 13.0 2.6e-227 740.4 13.0 1.0 1 lcl|FitnessBrowser__HerbieS:HSERO_RS19990 HSERO_RS19990 acetyl-CoA acetylt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__HerbieS:HSERO_RS19990 HSERO_RS19990 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 740.4 13.0 2.6e-227 2.6e-227 1 400 [] 2 400 .] 2 400 .] 1.00 Alignments for each domain: == domain 1 score: 740.4 bits; conditional E-value: 2.6e-227 TIGR02430 1 kevyivdairtpiGrygGslssvraddlaavplkallarnpsldaaaiddvilGcanqaGednrnvar 68 k+v+i+dairtpiGrygG+l vraddl+avpl+al++rnp++d++a+ddvi+GcanqaGednrnvar lcl|FitnessBrowser__HerbieS:HSERO_RS19990 2 KDVFICDAIRTPIGRYGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQAGEDNRNVAR 69 689***************************************************************** PP TIGR02430 69 maallaGlpvsvpgttvnrlcgsgldalglaaraikaGeadlviaGGvesmsrapfvlGkadsafsrs 136 m++llaGlp++vpg+t+nrlcgsg+dalg+aaraik+Ge++l+iaGGvesm+rapfv+Gkadsafsr+ lcl|FitnessBrowser__HerbieS:HSERO_RS19990 70 MSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVESMTRAPFVMGKADSAFSRQ 137 ******************************************************************** PP TIGR02430 137 akledttiGwrfvnpklkalyGvdsmpetaenvaeefgvsredqdafalrsqqrtaaaqakGffaeei 204 a ++dttiGwrfvn +k+ yGvd+mpetaenva +f+vsr+dqd+fa+rsq+++aaaqa+G +a+ei lcl|FitnessBrowser__HerbieS:HSERO_RS19990 138 AAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRADQDQFAVRSQAKAAAAQANGTLAQEI 205 ******************************************************************** PP TIGR02430 205 vpveikqkkGeetvvdkdehlraettlealaklkavvredgtvtaGnasGvndGaaalllaseeavkr 272 vpv+i+qkkG++ +v +deh+r t++eala+lk+vv++dg+vtaGnasGvndGa+alllas+eav++ lcl|FitnessBrowser__HerbieS:HSERO_RS19990 206 VPVVIPQKKGDPITVTQDEHPR-ATSMEALARLKGVVKPDGSVTAGNASGVNDGACALLLASAEAVEK 272 **********************.8******************************************** PP TIGR02430 273 hgltprarilaaasaGveprvmGlgpvpavkkllaraglsledldvielneafaaqalavlrelglad 340 ++l+praril++a+aGv+pr+mG+gp+pa kk+la++g++++++dvielneafa+q+lavlrelg+ad lcl|FitnessBrowser__HerbieS:HSERO_RS19990 273 YQLKPRARILGMATAGVAPRIMGMGPAPASKKVLAQLGMTIDQMDVIELNEAFASQGLAVLRELGVAD 340 ******************************************************************** PP TIGR02430 341 ddarvnpnGGaialGhplGasGarlvltalkqleksggryalatlciGvGqGialvierv 400 ddarvnpnGGaialGhplG+sGarlv+ta +qle++ggryal+t+ciGvGqGia+vierv lcl|FitnessBrowser__HerbieS:HSERO_RS19990 341 DDARVNPNGGAIALGHPLGMSGARLVTTATYQLERTGGRYALCTMCIGVGQGIAMVIERV 400 ***********************************************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (400 nodes) Target sequences: 1 (400 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.30 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory