Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate HSERO_RS08920 HSERO_RS08920 ABC transporter
Query= uniprot:A0A165KER0 (358 letters) >FitnessBrowser__HerbieS:HSERO_RS08920 Length = 655 Score = 165 bits (418), Expect = 3e-45 Identities = 100/329 (30%), Positives = 179/329 (54%), Gaps = 48/329 (14%) Query: 9 IIGAVALLVLPLILQSFGNAWVRIADLALLYVLLALGLNIVVGYAGLLDLGYVAFYAVGA 68 ++G VAL +P+++ + ++ + + L+Y +L GL+IVVGY G + LG+ + +G+ Sbjct: 10 LLGIVALAGIPMVVHN--PYYLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGS 67 Query: 69 YLFALMASPHLADNFAAFAAMFPNGLHTSLWIVIPVAALLAAFFGAMLGAPTLKLRGDYL 128 Y AA F G+ +++ +P A + A FGA+L P L++ G YL Sbjct: 68 Y--------------AAGVLFFKLGM--PIFVTVPAAIAVTAVFGAILALPALRVTGPYL 111 Query: 129 AIVTLGFGEIIRIFLNNLDHPVNLTNGPKGLGQIDSVKVFGLDLGKRLEVFGFDINSVTL 188 A+VTL FG II+I +N + LT GP G+ L K + G D S Sbjct: 112 AMVTLAFGTIIQILINEMTF---LTEGP-----------LGIKLQKPM--IGGDKMSEVQ 155 Query: 189 YYYLFLVLVVVSVIICYRLQDSRIGRAWMAIREDEIAAKAMGINTRNMKLLAFGMGASFG 248 ++YL VL+V+S+++ +R+ S +GRA+ A+R+ IA+ MG++ K+ AF + A+ Sbjct: 156 FFYLVAVLMVLSMLVVHRILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAALA 215 Query: 249 GVSGAMFGAFQGFVSPESFSLMESVMIVAMVVLGGIGHIPGVILGAVLLSALPEVLRYVA 308 G+SGA++ + ++SP +++ ++M + V++GG G ++GA+++ LP +L Sbjct: 216 GLSGALYAYSEEYISPNTYNFELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLS--- 272 Query: 309 GPLQAMTDGRLDSAILRQLLIALAMIIIM 337 D A+ RQ+ LA+I+++ Sbjct: 273 -----------DIALFRQIATVLAVIVVV 290 Lambda K H 0.328 0.144 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 461 Number of extensions: 18 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 358 Length of database: 655 Length adjustment: 34 Effective length of query: 324 Effective length of database: 621 Effective search space: 201204 Effective search space used: 201204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory