GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AZOBR_RS08245 in Escherichia coli BW25113

Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate 17516 b3455 leucine/isoleucine/valine transporter subunit (NCBI)

Query= uniprot:G8ALJ0
         (294 letters)



>FitnessBrowser__Keio:17516
          Length = 255

 Score =  262 bits (669), Expect = 7e-75
 Identities = 140/260 (53%), Positives = 186/260 (71%), Gaps = 9/260 (3%)

Query: 10  PLLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPTVGRLT 69
           PLL+V  L MRFGGL+AVN+V+      EI ++IGPNGAGKTT+FNC+TGFY PT G + 
Sbjct: 4   PLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGKTTVFNCLTGFYKPTGGTIL 63

Query: 70  LRHADGKEFLLERMPGYRISQKASVARTFQNIRLFGGMSVLENLIVAQHNKLIRASGFSI 129
           LR        LE +PG +I+ +  V RTFQ++RLF  M+V+ENL+VAQH +L +   FS 
Sbjct: 64  LRDQH-----LEGLPGQQIA-RMGVVRTFQHVRLFREMTVIENLLVAQHQQL-KTGLFS- 115

Query: 130 AGLLGLPSYTRTEREAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMCTEPVM 189
            GLL  PS+ R + EA+D A  WL+R+ LLE A+ +A NL YG QRRLEIAR M T+P +
Sbjct: 116 -GLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQRRLEIARCMVTQPEI 174

Query: 190 LCLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDYGRKIS 249
           L LDEPAAGLNP+E+ EL +L+  +R+ H   +LLIEHDM +VM ISD + V++ G  ++
Sbjct: 175 LMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVMGISDRIYVVNQGTPLA 234

Query: 250 DGDPAFVKNDPAVIRAYLGE 269
           +G P  ++N+P VIRAYLGE
Sbjct: 235 NGTPEQIRNNPDVIRAYLGE 254


Lambda     K      H
   0.319    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 217
Number of extensions: 7
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 294
Length of database: 255
Length adjustment: 25
Effective length of query: 269
Effective length of database: 230
Effective search space:    61870
Effective search space used:    61870
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory