GapMind for catabolism of small carbon sources

 

Aligments for a candidate for araV in Escherichia coli BW25113

Align AraV, component of Arabinose, fructose, xylose porter (characterized)
to candidate 14400 b0262 putative ATP-binding component of a transport system (VIMSS)

Query= TCDB::Q97UF2
         (371 letters)



>lcl|FitnessBrowser__Keio:14400 b0262 putative ATP-binding component
           of a transport system (VIMSS)
          Length = 348

 Score =  212 bits (540), Expect = 1e-59
 Identities = 114/288 (39%), Positives = 181/288 (62%), Gaps = 10/288 (3%)

Query: 4   IRVENLSKIFKKGKTEVKAVDNVSITIDSGMAFGVLGPSGHGKTTFLRLIAGLEEPTSGY 63
           + + N++K F  G   V  +DN+++TI  G    +LGPSG GKTT LRL+AGLE+P+ G 
Sbjct: 7   VELRNVTKRF--GSNTV--IDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQ 62

Query: 64  IYFDNEAVSSPRRVMMSPEKRGIAMVFQNWALYPNMTVFDNIAFPLKLAKVPKDKIENKV 123
           I+ D E V+       S ++R I MVFQ++AL+P+M++ +N+ + LK+  VP+ +++ +V
Sbjct: 63  IFIDGEDVTH-----RSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVPRAELKARV 117

Query: 124 KEVSEELGLSGVLNRYPKELSGGQMQRTAIARALVKDPKVLLLDEPFSNLDAQIRESARA 183
           KE    + L G  +R+  ++SGGQ QR A+ARAL+  PKVLL DEP SNLDA +R S R 
Sbjct: 118 KEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRD 177

Query: 184 LVRKIQRERKLTTLIVSHDPADIFAIANKAGVIVNGKFAQIGTPTEIYEYPATDLIARLT 243
            +R++Q++  +T+L V+HD ++ FA+++   V+  G   QIG+P ++Y  PA+  +A   
Sbjct: 178 KIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFM 237

Query: 244 GEINLIQAKIIENNAIIANLKVPLNNMELKGQSNIVIGLRPDDLTLSD 291
           G+ NL  A   +    I    +P   +    Q   ++G+RP+ +TLSD
Sbjct: 238 GDANLFPATFSDGYVDIYGYHLP-RPLHFGTQGEGMVGVRPEAITLSD 284


Lambda     K      H
   0.317    0.136    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 264
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 371
Length of database: 348
Length adjustment: 29
Effective length of query: 342
Effective length of database: 319
Effective search space:   109098
Effective search space used:   109098
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory