GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gyaR in Escherichia coli BW25113

Align Glyoxylate reductase; EC 1.1.1.26 (uncharacterized)
to candidate 15502 b1380 D-lactate dehydrogenase (NCBI)

Query= curated2:B1L765
         (332 letters)



>lcl|FitnessBrowser__Keio:15502 b1380 D-lactate dehydrogenase (NCBI)
          Length = 329

 Score =  167 bits (424), Expect = 3e-46
 Identities = 105/332 (31%), Positives = 170/332 (51%), Gaps = 21/332 (6%)

Query: 1   MKPRVFVTREIPERGLSKIEEHFELDLWKDEAPPSKKVIIERVKDCDALVSLLTDPIDAE 60
           MK  V+ T++  ++ L ++ E F  +L   +   ++K   +    C+A+   + D     
Sbjct: 1   MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTA-KTANGCEAVCIFVNDDGSRP 59

Query: 61  VFEAAPK--LRIVAQYAVGYDNIDVKEATKRGIYVTNTPGVLTETTADFAFALLMAAARR 118
           V E   K  ++ +A    G++N+D+  A + G+ V   P    E  A+ A  ++M   RR
Sbjct: 60  VLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRR 119

Query: 119 VVEADRYVREGKWKVAWHPMMMLGYDVYGRTLGIVGMGRIGAAVARRAKGFGMRILYYDS 178
           +  A +  R+  + +      + G+ +YG+T G++G G+IG A+ R  KGFGMR+L +D 
Sbjct: 120 IHRAYQRTRDANFSLEG----LTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDP 175

Query: 179 IRREDFEKELGVEYVPLEKLLEESDFVSLHVPLTEETYHMIGEEQLRRMKRTAILVNTSR 238
                   ELGVEYV L  L  ESD +SLH PLT E YH++ E    +MK   ++VNTSR
Sbjct: 176 YPSAA-ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSR 234

Query: 239 GKVVDQKALYKALKEGWIAGAGLDVFEQE----------PIPPDDPLLKL---ENVVLAP 285
           G ++D +A  +ALK   I   G+DV+E E           +  DD   +L    NV+   
Sbjct: 235 GALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTG 294

Query: 286 HAASASHETRSRMAEMVAENLIAFKRGEIPPN 317
           H A  + E  + +++   +NL   ++GE  PN
Sbjct: 295 HQAFLTAEALTSISQTTLQNLSNLEKGETCPN 326


Lambda     K      H
   0.319    0.137    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 230
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 332
Length of database: 329
Length adjustment: 28
Effective length of query: 304
Effective length of database: 301
Effective search space:    91504
Effective search space used:    91504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory