Align Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale)
to candidate 17511 b3450 ATP-binding component of sn-glycerol 3-phosphate transport system (VIMSS)
Query= uniprot:D4GP39 (383 letters) >FitnessBrowser__Keio:17511 Length = 356 Score = 308 bits (789), Expect = 2e-88 Identities = 175/366 (47%), Positives = 237/366 (64%), Gaps = 16/366 (4%) Query: 1 MARLTLDDVTKVYTDEGGGDIVAVEEISLDIDDGEFLVLVGPSGCGKSTTLRMMAGLETV 60 MA L L VTK + G ++ ++LD+ DGEF+V+VGPSGCGKST LRM+AGLE V Sbjct: 1 MAGLKLQAVTKSWD----GKTQVIKPLTLDVADGEFIVMVGPSGCGKSTLLRMVAGLERV 56 Query: 61 TEGELRLEDRVLNGVSAQDRDIAMVFQSYALYPHKSVRGNMSFGLEESTGLPDDEIRQRV 120 TEG++ + D+ + + +DR IAMVFQ+YALYPH SV NM++GL+ G+ +I +RV Sbjct: 57 TEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK-IRGMGKQQIAERV 115 Query: 121 EETTDMLGISDLLDRKPGQLSGGQQQRVALGRAIVRDPEVFLMDEPLSNLDAKLRAEMRT 180 +E +L + LL R+P +LSGGQ+QRVA+GRAIVRDP VFL DEPLSNLDAKLR +MR Sbjct: 116 KEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRL 175 Query: 181 ELQRLQGELGVTTVYVTHDQTEAMTMGDRVAVLDDGELQQVGTPLDCYHRPNNLFVAGFI 240 ELQ+L L T++YVTHDQ EAMT+ RV V++ G +Q+GTP++ Y +P +LFVA FI Sbjct: 176 ELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFI 235 Query: 241 GEPSMNLFDGSLS--GDTFRGD-GFDYPLSGATRDQLGGASGLTLGIRPEDVTVGERRSG 297 G P+MNL G ++ G F D G + PL+G R G +TLGIRPE + + + G Sbjct: 236 GSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAG--RKMTLGIRPEHIALSSQAEG 293 Query: 298 QRTFDAEVVVVEPQGNENAVHLRFVDGDEGTQFTATTTGQSRVEAGDRTTVSFPEDAIHL 357 + +E G +N H R+ G++ + Q R AG + E+ +HL Sbjct: 294 GVPMVMD--TLEILGADNLAHGRW--GEQ--KLVVRLAHQERPTAGSTLWLHLAENQLHL 347 Query: 358 FDGETG 363 FDGETG Sbjct: 348 FDGETG 353 Lambda K H 0.316 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 427 Number of extensions: 26 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 356 Length adjustment: 30 Effective length of query: 353 Effective length of database: 326 Effective search space: 115078 Effective search space used: 115078 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory