Align Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale)
to candidate 18063 b4035 fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI)
Query= uniprot:D4GP39 (383 letters) >FitnessBrowser__Keio:18063 Length = 371 Score = 293 bits (751), Expect = 4e-84 Identities = 165/366 (45%), Positives = 224/366 (61%), Gaps = 19/366 (5%) Query: 1 MARLTLDDVTKVYTDEGGGDIVAVEEISLDIDDGEFLVLVGPSGCGKSTTLRMMAGLETV 60 MA + L +VTK + G++V ++I+LDI +GEF+V VGPSGCGKST LRM+AGLET+ Sbjct: 1 MASVQLQNVTKAW-----GEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIAGLETI 55 Query: 61 TEGELRLEDRVLNGVSAQDRDIAMVFQSYALYPHKSVRGNMSFGLEESTGLPDDEIRQRV 120 T G+L + ++ +N +R + MVFQSYALYPH SV NMSFGL+ + G + I QRV Sbjct: 56 TSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKLA-GAKKEVINQRV 114 Query: 121 EETTDMLGISDLLDRKPGQLSGGQQQRVALGRAIVRDPEVFLMDEPLSNLDAKLRAEMRT 180 + ++L ++ LLDRKP LSGGQ+QRVA+GR +V +P VFL+DEPLSNLDA LR +MR Sbjct: 115 NQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEPLSNLDAALRVQMRI 174 Query: 181 ELQRLQGELGVTTVYVTHDQTEAMTMGDRVAVLDDGELQQVGTPLDCYHRPNNLFVAGFI 240 E+ RL LG T +YVTHDQ EAMT+ D++ VLD G + QVG PL+ YH P + FVAGFI Sbjct: 175 EISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKPLELYHYPADRFVAGFI 234 Query: 241 GEPSMNLFDGSLSGDTFRGDGFDYPLSG--------ATRDQLGGASGLTLGIRPEDVTVG 292 G P MN ++ + P+ +RD GA+ ++LGIRPE + Sbjct: 235 GSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQVGAN-MSLGIRPEHLLPS 293 Query: 293 ERRSGQRTFDAEVVVVEPQGNENAVHLRFVDGDEGTQFTATTTGQSRVEAGDRTTVSFPE 352 + + EV VVE GNE +H++ + + VE G + P Sbjct: 294 D--IADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV--VLVEEGATFAIGLPP 349 Query: 353 DAIHLF 358 + HLF Sbjct: 350 ERCHLF 355 Lambda K H 0.316 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 393 Number of extensions: 24 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 371 Length adjustment: 30 Effective length of query: 353 Effective length of database: 341 Effective search space: 120373 Effective search space used: 120373 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory