Alignments for a candidate for rocE in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for rocE in Escherichia coli BW25113

Align Amino-acid permease RocE (characterized)
to candidate 14258 b0112 aromatic amino acid transporter (NCBI)

Query= SwissProt::P39137
         (467 letters)



>FitnessBrowser__Keio:14258
          Length = 457

 Score =  311 bits (797), Expect = 3e-89
 Identities = 161/451 (35%), Positives = 261/451 (57%), Gaps = 7/451 (1%)

Query: 1   MNTNQDNGNQLQRTMKSRHLFMISLGGVIGTGFFLGTGFTINQAGPLGAVLSYLVGGFIM 60
           M   Q +G QL+R +K+RH+ +I+LGG IGTG FLG+   I  AGP G +L Y + GFI 
Sbjct: 1   MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGP-GIILGYAIAGFIA 59

Query: 61  FLTMLCLGELAVAFPVSGSFQTYATKFISPAFGFAFGWLYWLGWAVTCAIEFLSAGQLMQ 120
           FL M  LGE+ V  PV+GSF  +A K+     GFA GW YW+ + +    E  + G+ +Q
Sbjct: 60  FLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQ 119

Query: 121 RWFPHIDVWIWCLVFAALMFILNAITTKAFAESEFWFSGIKILIILLFIILGGAAMFGLI 180
            W+P I  W+   VF  ++  +N    K F E EFWF+ IK++ ++  II GG  +F   
Sbjct: 120 FWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSG- 178

Query: 181 DLKGGEQAPFLTHFYEDGLFPNGIKAMLITMITVNFAFQGTELIGVAAGESEDPEKTIPR 240
              GG QA     + + G  P+G   +++ M  + F+F G EL+G+ A E+++PE++IP+
Sbjct: 179 --NGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPK 236

Query: 241 SIKQTVWRTLVFFVLSIIVIAGMIPWKQAGVVESPFVAVFEQIGIPYAADIMNFVILIAL 300
           +  Q ++R L+F++ S+ V+  ++PW +     SPFV +F ++G  + A+ +N V+L A 
Sbjct: 237 ATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAA 296

Query: 301 LSVANSGLYASTRILYAMANEGQAFKALGKTNQRGVPMYSLIVTMAVACLSLLTKFAQAE 360
           LSV NS +Y ++R+L+ +A +G A KAL   ++RGVP+ +++V+  V  L +L  +   E
Sbjct: 297 LSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPE 356

Query: 361 TVYMVLLSLAGMSAQVGWITISLSQIMFRRKYIREGGKIEDLKFKTPLYPVLPLIGLTLN 420
           + + +L++L   +  + W  ISL+ + FRR    +G      +F   LYP+   I L   
Sbjct: 357 SAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG---VVTRFPALLYPLGNWICLLFM 413

Query: 421 TVVLISLAFDPEQRIALYCGVPFMIICYIIY 451
             VL+ +   P   I++Y    ++I+  I Y
Sbjct: 414 AAVLVIMLMTPGMAISVYLIPVWLIVLGIGY 444


Lambda     K      H
   0.329    0.142    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 518
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 467
Length of database: 457
Length adjustment: 33
Effective length of query: 434
Effective length of database: 424
Effective search space:   184016
Effective search space used:   184016
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources