Align NatH, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (characterized)
to candidate 14790 b0653 glutamate and aspartate transporter subunit (NCBI)
Query= TCDB::Q8YPM7 (381 letters) >FitnessBrowser__Keio:14790 Length = 224 Score = 124 bits (310), Expect = 3e-33 Identities = 73/210 (34%), Positives = 122/210 (58%), Gaps = 8/210 (3%) Query: 174 LWNGLLLTLLMAAISIVLSFPIGVLLALGRTSNLPVVRWFSILYIEIVRGVPLIGILFLA 233 L +GL++TL + ++V+ G +LA+ R S+ V WF+ Y+ + R +PL+ +L Sbjct: 16 LLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWF 75 Query: 234 QVMLPLFFAADVRLD-----RVLRAIAGLVLFSAAYMAENVRGGLQAVSRGQVEAAKALG 288 +++P F + L R++ A+ +F AAY +E +R G+Q++SRGQ AA ALG Sbjct: 76 YLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALG 135 Query: 289 LNTFFVVLLIVLPQALRAVIPALVGQFIGLFKDTSLLSLVGLVELTGIARSILAQPQFIG 348 + + + LI+LPQA RA++P L+ Q I LF+DTSL+ ++ L + A +I + G Sbjct: 136 MTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADFFRTASTIGERD---G 192 Query: 349 RYAEVYLFIGLIYWLFCYSMSLASRRLERQ 378 E+ LF G +Y++ S SL L+R+ Sbjct: 193 TQVEMILFAGFVYFVISLSASLLVSYLKRR 222 Lambda K H 0.332 0.145 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 234 Number of extensions: 12 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 381 Length of database: 224 Length adjustment: 26 Effective length of query: 355 Effective length of database: 198 Effective search space: 70290 Effective search space used: 70290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory