GapMind for catabolism of small carbon sources

 

Aligments for a candidate for cbtF in Escherichia coli BW25113

Align CbtF, component of Cellobiose and cellooligosaccharide porter (characterized)
to candidate 17601 b3540 dipeptide transporter (NCBI)

Query= TCDB::Q97VF4
         (324 letters)



>lcl|FitnessBrowser__Keio:17601 b3540 dipeptide transporter (NCBI)
          Length = 334

 Score =  195 bits (496), Expect = 1e-54
 Identities = 108/307 (35%), Positives = 182/307 (59%), Gaps = 14/307 (4%)

Query: 16  KVGLFK-KRKFYALKDVSLSMNQGDLLIVLGESGAGKTTLGRVIVGLQKPTSGEVVYDGY 74
           K G+F  +R   AL  VS ++ +G  L V+GESG GK+TLGR++  ++ PT GE+ Y G 
Sbjct: 25  KKGMFAPERLVKALDGVSFNLERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQ 84

Query: 75  NIWKNKRKIFKKYRKDVQLIPQDPYSTLPFNKTVEEILVAPILRWEKINKDELRKRLINL 134
           ++ K+  +  K  R+ +Q++ Q+PY +L   K V +IL  P+L    ++K++ R++ +++
Sbjct: 85  DLLKHDPQAQKLRRQKIQIVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSM 144

Query: 135 LELVKLTPAEEFLGKYPHQLSGGQKQRLSIARSLSVNPRIIVADEPVTMVDASLRIGILN 194
           +  V L    E   +YPH  SGGQ+QR++IAR L ++P +++ADEPV+ +D S+R  +LN
Sbjct: 145 MAKVGL--KTEHYDRYPHMFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLN 202

Query: 195 TLAEIKNRLNLTMVFITHDIPIARYFYHLFDKGNTIVMFAGRIVERADLEEILKDPLHPY 254
            + +++  L L+ VFI+HD+ +     H+ D+   +VM+ GR VE+   ++I  +P HPY
Sbjct: 203 LMMDLQQELGLSYVFISHDLSVVE---HIADE--VMVMYLGRCVEKGTKDQIFNNPRHPY 257

Query: 255 TNDLIKLTPSIDNLYKEINVKINYE-----RVEKGCPYRLRCPFAMDICKNEEPKLFKYS 309
           T  L+  TP ++   +   +K++ E         GC +  RC      C   +P+L  Y 
Sbjct: 258 TQALLSATPRLNPDDRRERIKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYG 317

Query: 310 HE-VACF 315
            + VACF
Sbjct: 318 GQLVACF 324


Lambda     K      H
   0.321    0.141    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 308
Number of extensions: 15
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 324
Length of database: 334
Length adjustment: 28
Effective length of query: 296
Effective length of database: 306
Effective search space:    90576
Effective search space used:    90576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory