Aligments for a candidate for astD in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Aligments for a candidate for astD in Escherichia coli BW25113

Align N-succinylglutamate 5-semialdehyde dehydrogenase 1; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase 1; SGSD 1 (uncharacterized)
to candidate 15420 b1300 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI)

Query= curated2:Q9AAL5
         (472 letters)



>FitnessBrowser__Keio:15420
          Length = 495

 Score =  202 bits (513), Expect = 3e-56
 Identities = 152/446 (34%), Positives = 220/446 (49%), Gaps = 12/446 (2%)

Query: 10  DPYTGEAIADFAVNDARSIDAACHSARAAF--AEWAMTPLAERRAIALRFAETVRARREE 67
           DP T   +A  A   +  ID A  +AR  F   +W+++  A+R+A+  + A+ + A  EE
Sbjct: 41  DPVTQAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEE 100

Query: 68  IATLIARETGKPMWEALTEADSVAAK-VAISIRAQDERAGERSEPMADATARLAHRPHGV 126
           +A L   +TGKP+  +L +    AA+ +     A D+  GE +   +   A +   P GV
Sbjct: 101 LALLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV 160

Query: 127 LAVIGPFNFPMHLANGHIVPALLAGNAVVFKPSEKTPACGQLMGELWRAAGLPDHVLTIV 186
           +A I P+NFP+ L    + PAL AGN+V+ KPSEK+P     +  L + AGLPD VL +V
Sbjct: 161 IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLNVV 220

Query: 187 IG-GGEAGEALVRHEALDGVLFTGGVQAGRAIHRALADAPHKILALELGG-NAPLVVWDV 244
            G G EAG+AL RH  +D + FTG  + G+ + +   D+  K + LE GG +A +V  D 
Sbjct: 221 TGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIVFADC 280

Query: 245 ADIEAAAHLIVQSAYVTAGQRCTCARRLILPEGARGDALLEALTMLMDRLVIGGPFQSPA 304
            D++ AA       +   GQ C    RL+L E    D  L  L         G P   PA
Sbjct: 281 PDLQQAASATAAGIFYNQGQVCIAGTRLLLEESI-ADEFLALLKQQAQNWQPGHPL-DPA 338

Query: 305 PFMGPVIDAHAAAQVLAAQDRMTADGGRPLRLAAVREARSALLSPGLIELTD--APLRDE 362
             MG +ID   A  V +      + G   L L       +A + P +    D  A L  E
Sbjct: 339 TTMGTLIDCAHADSVHSFIREGESKG--QLLLDGRNAGLAAAIGPTIFVDVDPNASLSRE 396

Query: 363 EIFGPLLQVRRAADFDAALALANATRFGLAAGLISDDEALYRRFWTSVRAGIVNWNRPTT 422
           EIFGP+L V R    + AL LAN +++GL A + + D +   R    ++AG V  N    
Sbjct: 397 EIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVFVNNYND 456

Query: 423 GASSAAPFGGVGGSGNHRPSAYYAAD 448
           G     PFGG   SGN R  + +A +
Sbjct: 457 G-DMTVPFGGYKQSGNGRDKSLHALE 481


Lambda     K      H
   0.320    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 544
Number of extensions: 24
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 472
Length of database: 495
Length adjustment: 34
Effective length of query: 438
Effective length of database: 461
Effective search space:   201918
Effective search space used:   201918
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources