Alignments for a candidate for nupA in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for nupA in Escherichia coli BW25113

Align Purine/cytidine ABC transporter ATP-binding protein, component of General nucleoside uptake porter, NupABC/BmpA (transports all common nucleosides as well as 5-fluorocytidine, inosine, deoxyuridine and xanthosine) (Martinussen et al., 2010) (Most similar to 3.A.1.2.12). NupA is 506aas with two ABC (C) domains. NupB has 8 predicted TMSs, NupC has 9 or 10 predicted TMSs in a 4 + 1 (or 2) + 4 arrangement (characterized)
to candidate 16258 b2149 fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI)

Query= TCDB::A2RKA7
         (506 letters)



>FitnessBrowser__Keio:16258
          Length = 506

 Score =  302 bits (773), Expect = 2e-86
 Identities = 169/494 (34%), Positives = 283/494 (57%), Gaps = 4/494 (0%)

Query: 2   ANETVIQMIDVTKRFGDFVANDKVNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEG 61
           + E +++M  + K F    A D VNL+++   IHAL+GENGAGKSTL+  L G+ +   G
Sbjct: 9   SGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSG 68

Query: 62  EVHVKGKLENIDSPSKAANLGIGMVHQHFMLVDAFTVTENIILGNEVTKGINLDLKTAKK 121
            +  +GK  +  S  +A   GI MVHQ   LV   +V +N+ LG   TKG+ +D     +
Sbjct: 69  TILFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYPTKGMFVDQDKMYR 128

Query: 122 KILELSERYGLSVEPDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITEL 181
           +   + +   + ++P A +  +SV Q Q +EI K     A I+I DEPT+ LT  E+  L
Sbjct: 129 ETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHL 188

Query: 182 MQIMKNLIKEGKSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAELMVGR 241
             I++ L + G  I+ I+HK++EI  + D +TV+R G+ I T  L   T  ++  +MVGR
Sbjct: 189 FTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVGR 248

Query: 242 SVSFITEKAAAQPKDVVLEIKDLNIKESRGSLKVKGLSLDVRAGEIVGVAGIDGNGQTEL 301
           S++        +P +V+LE+++L    S     ++ +S D+  GEI+G+AG+ G  +T++
Sbjct: 249 SLNQRFPDKENKPGEVILEVRNLT---SLRQPSIRDVSFDLHKGEILGIAGLVGAKRTDI 305

Query: 302 VKAITGLTKVDSGSIKLHNKDITNQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENIALQ 361
           V+ + G+ +  +G+I LH K I N    +        V E+R   G+   + +  N +L 
Sbjct: 306 VETLFGIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYLDIGFN-SLI 364

Query: 362 TYYKPPMSKYGFLDYNKINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREIDRN 421
           +  +   +K G LD +++ S  + +++   V+  G      SLSGGNQQK II R +   
Sbjct: 365 SNIRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQ 424

Query: 422 PDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHDGQI 481
           P++L++ +PTRG+DVGA   I++ + +   +GK +++IS E+ E+L ++DRI V+ +G +
Sbjct: 425 PEILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMSNGLV 484

Query: 482 QGIVSPETTTKQEL 495
            GIV  +TTT+ E+
Sbjct: 485 SGIVDTKTTTQNEI 498



 Score = 73.9 bits (180), Expect = 1e-17
 Identities = 60/225 (26%), Positives = 107/225 (47%), Gaps = 17/225 (7%)

Query: 25  VNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGEVHVKGKLENIDSPSKAANLGIG 84
           V+ +L KGEI  + G  GA ++ ++  L G+ E S G + + GK  N  + ++A N G  
Sbjct: 282 VSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANEAINHGFA 341

Query: 85  MVHQHFMLVDAFTVTE---NIILGN------EVTKGINLDLKTAKKKILELSERYGLSVE 135
           +V +       +   +   N ++ N      +V    N  +K+  + +++      + V+
Sbjct: 342 LVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVID-----SMRVK 396

Query: 136 PDA---LIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITELMQIMKNLIKEG 192
                  I  +S G QQ+V I + L    +IL+ DEPT  +      E+ Q++  L K+G
Sbjct: 397 TPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKG 456

Query: 193 KSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAEL 237
           K II+I+ ++ E+  + DRI V+  G     V+    T  E+  L
Sbjct: 457 KGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRL 501


Lambda     K      H
   0.315    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 631
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 506
Length adjustment: 34
Effective length of query: 472
Effective length of database: 472
Effective search space:   222784
Effective search space used:   222784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources