GapMind for catabolism of small carbon sources

 

ethanol catabolism in Escherichia coli BW25113

Best path

etoh-dh-nad, adh, ackA, pta

Also see fitness data for the top candidates

Rules

Overview: Ethanol can pass through biological membranes, so no transporter is required. Ethanol degradation in GapMind is based on MetaCyc pathways ethanol degradation I (oxidation to acetyl-CoA, link) and II (oxidation to acetate and activation, link). Pathways III (with ethanol monooxygenase, link) and IV (with ethanol peroxidase, link) are not reported to occur in prokaryotes and are not included.

10 steps (6 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
etoh-dh-nad ethanol dehydrogenase (NAD(P)) b1241 b0356
adh acetaldehyde dehydrogenase (not acylating) b1746 b1241
ackA acetate kinase b3115 b2296
pta phosphate acetyltransferase b2297 b2458
Alternative steps:
acs acetyl-CoA synthetase, AMP-forming b4069 b0335
adhAqn ethanol dehydrogenase (quinone), subunit I
adhBqn ethanol dehydrogenase (quinone), subunit II
adhSqn ethanol dehydrogenase (quinone), subunit III
ald-dh-CoA acetaldehyde dehydrogenase, acylating b1241 b2455
etoh-dh-c ethanol dehydrogenase (cytochrome c)

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory