Alignments for a candidate for fruII-ABC in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruII-ABC in Escherichia coli BW25113

Align The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system (characterized)
to candidate 16276 b2167 fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI)

Query= TCDB::P71012
         (635 letters)



>FitnessBrowser__Keio:16276
          Length = 563

 Score =  404 bits (1039), Expect = e-117
 Identities = 211/473 (44%), Positives = 308/473 (65%), Gaps = 17/473 (3%)

Query: 162 AAPAPAGKGKILAVTACPTGIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQE 221
           A  A +G  +++AVTACPTG+AHTFMAA+A++ +AK+ G  +KVET GS G  + +T +E
Sbjct: 96  APVAASGPKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEE 155

Query: 222 IEDAPAIIVAADKQVEMERFKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSA 281
           +  A  +IVAAD +V++ +F GK + +      +++  + ++KA+ +  P Y+ +G    
Sbjct: 156 VAAADLVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATP-YEPAGKAQT 214

Query: 282 ASNDDEEAKGKSGSGIGNTFYKHLMSGVSNMLPFVVGGGILVAISFFWGIHSADPNDPSY 341
           A+   E  K  +G+      Y+HL++GVS MLP VV GG+ +A+SF +GI +    +P  
Sbjct: 215 ATT--ESKKESAGA------YRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAF--KEPG- 263

Query: 342 NTFAAALNFIGGDNALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLIA 401
            T AAAL  IGG +A  L+V VLAG+IA SIADRPG  PG++GG +A    +GF+GG+IA
Sbjct: 264 -TLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIA 322

Query: 402 GFLAGYVVILLKKVFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTN 461
           GFLAGY+  L+      +PQS++ LKP+LI PL    + G+ M +++  PVA  +  LT+
Sbjct: 323 GFLAGYIAKLISTQLK-LPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTH 381

Query: 462 WLESLGTGNLVLMGIILGGMMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPP 521
           WL+++GT N VL+G ILGGMM  DMGGP+NKAA+ FG+ ++    Y P AAIMA GMVPP
Sbjct: 382 WLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPP 441

Query: 522 LGIALATTIFRNKFTQRDREAGITCYFMGAAFVTEGAIPFAAADPLRVIPAAVVGAAVAG 581
           L + LAT + R KF +  +E G     +G  F++EGAIPFAA DP+RV+P  +VG A+ G
Sbjct: 442 LAMGLATMVARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTG 501

Query: 582 GLTEFFRVTLPAPHGGVFVAFITN--HPML-YLLSIVIGAVVMAIILGIVKKP 631
            ++      L APHGG+FV  I     P+L YL++I+ G +V  +    +K+P
Sbjct: 502 AISMAIGAKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRP 554


Lambda     K      H
   0.320    0.137    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 831
Number of extensions: 42
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 563
Length adjustment: 37
Effective length of query: 598
Effective length of database: 526
Effective search space:   314548
Effective search space used:   314548
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources