Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate 16488 b2383 fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI)
Query= reanno::pseudo5_N2C3_1:AO356_17540 (837 letters) >FitnessBrowser__Keio:16488 Length = 831 Score = 343 bits (879), Expect = 3e-98 Identities = 215/659 (32%), Positives = 342/659 (51%), Gaps = 22/659 (3%) Query: 183 GLHARPAALVRQTAQGFKSRSQL--HFSGKSASCDSVMGMMGLAITEQAQVHVSCRGSDA 240 GLHARPA +++ ++S H A S + ++G ++ GSD Sbjct: 13 GLHARPAWELKEQCSQWQSEITFINHRQNAKADAKSSLALIGTGTLFNDSCSLNISGSDE 72 Query: 241 EAALQALLTTLSTALVE-------EAHASAPPPEPPRANAEEGVLHGVCAAPGLVTGPLV 293 E A + L + ++ +A +A P + +L+G A G+ G L Sbjct: 73 EQARRVLEEYIQVRFIDSDSVQPTQAELTAHPLPRSLSRLNPDLLYGNVLASGVGVGTLT 132 Query: 294 RLSGIQLPEDIGGHAIEEQRQRLSDALAQVRGEIHLTLEHARARQHRDEEAIFSAHLALL 353 L L A + RL +LA + +++ L + + + I SAHL+L+ Sbjct: 133 LLQSDSLDSYRAIPASAQDSTRLEHSLATLAEQLNQQLRE----RDGESKTILSAHLSLI 188 Query: 354 EDPVLL-DAADLFIEQGSAAPHAWSRSIDTQCQVLQQLGSTLLAERANDLRDLRQRVLRV 412 +D + L EQ A +++ C L S L ER +D+RD+ +++L + Sbjct: 189 QDDEFAGNIRRLMTEQHQGLGAAIISNMEQVCAKLSASASDYLRERVSDIRDISEQLLHI 248 Query: 413 LLGEAW---QFDVAAGAIVAAQELTPSDLLQLSAQGVAGVCMVEGGATSHVAILARGKGL 469 E + + I+ A++LTPS L L + +AG+ + + G TSH ILAR + Sbjct: 249 TWPELKPRNKLVLEKPTILVAEDLTPSQFLSLDLKNLAGMILEKTGRTSHTLILARASAI 308 Query: 470 PCLVALGDELLAQEQGQAVVLDADGGRLELTPTVERLAQVRQAQTRRTALRAQQQSLAHT 529 P L L + +A+ GQ VLDA G L + P + AQT + QQ A Sbjct: 309 PVLSGLPLDAIARYAGQPAVLDAQCGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQ 368 Query: 530 PARTVDGVEVEVAANVASSAEAGESLANGADGVGLLRTEFLFVDRHTAPDEEEQRQAYQA 589 A + D +++AAN+ ++ EA + ANGA+GVGL RTE L++DR +APDE+EQ +AYQ Sbjct: 369 LAYSRDNKRIDIAANIGTALEAPGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQ 428 Query: 590 VLEAMGDKPVIIRTIDVGGDKQLDYLPLPSEANPVLGLRGIRLAQVRPDLLDQQLRALLQ 649 VL A GDKP+I RT+D+GGDK + YL +P E NP LG R +R+ L QLRA+L+ Sbjct: 429 VLLAAGDKPIIFRTMDIGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILR 488 Query: 650 TRPLDRCRILLPMVTEVDELLHIRKRLDALGSEL---GL--SERPQLGVMVEVPAAALLA 704 ++++PMV +D++L ++ + EL GL +E LG+MVEVP+ + Sbjct: 489 AASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYII 548 Query: 705 EQLAEHADFLSIGTNDLSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWV 764 + + DF SIG+ND++QY A+DR++ ++ + + P+ LR++ Q A + G+WV Sbjct: 549 DHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWV 608 Query: 765 GVCGALASDPLATPVLIGLGVRELSVSPPQIGEIKDRVRHLDAAQCARLSNELLNLGSA 823 G+CG L + P+L+GLG+ ELS+S P+I +K ++R LD+ C L+ + SA Sbjct: 609 GICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSA 667 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1234 Number of extensions: 50 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 837 Length of database: 831 Length adjustment: 42 Effective length of query: 795 Effective length of database: 789 Effective search space: 627255 Effective search space used: 627255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory