GapMind for catabolism of small carbon sources

 

D-glucose catabolism in Escherichia coli BW25113

Best path

mglA, mglB, mglC, glk

Also see fitness data for the top candidates

Rules

Overview: In most bacteria, glucose is consumed via glucose 6-phosphate, which is a central metabolic intermediate. It can also be oxidized to 2-ketogluconate in the periplasm before uptake and conversion to gluconate 6-phosphate (link). Periplasmic oxidation to gluconate, uptake, and phosphorylation by gnuK is also a potential path to gluconate-6-phosphate, but is not included in GapMind because it is not known to be the major path for glucose utilization in a prokaryote.

39 steps (22 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
mglA glucose ABC transporter, ATP-binding component (MglA) b2149 b3567
mglB glucose ABC transporter, substrate-binding component b2150 b3566
mglC glucose ABC transporter, permease component (MglC) b2148 b3750
glk glucokinase b2388 b0394
Alternative steps:
aglE' glucose ABC transporter, substrate-binding component (AglE)
aglF' glucose ABC transporter, permease component 1 (AglF)
aglG' glucose ABC transporter, permease component 2 (AglG) b4032
aglK' glucose ABC transporter, ATPase component (AglK) b4035 b3450
bglF glucose PTS, enzyme II (BCA components, BglF) b3722 b2715
crr glucose PTS, enzyme IIA b2417 b0679
eda 2-keto-3-deoxygluconate 6-phosphate aldolase b1850 b4477
edd phosphogluconate dehydratase b1851 b3771
gadh1 gluconate 2-dehydrogenase flavoprotein subunit
gadh2 gluconate 2-dehydrogenase cytochrome c subunit
gadh3 gluconate 2-dehydrogenase subunit 3
gdh quinoprotein glucose dehydrogenase b0124 b0837
glcS glucose ABC transporter, substrate-binding component (GlcS)
glcT glucose ABC transporter, permease component 1 (GlcT)
glcU glucose ABC transporter, permease component 2 (GlcU)
glcU' Glucose uptake protein GlcU
glcV glucose ABC transporter, ATPase component (GclV) b0262 b4035
gnl gluconolactonase
gtsA glucose ABC transporter, substrate-binding component (GtsA)
gtsB glucose ABC transporter, permease component 1 (GtsB)
gtsC glucose ABC transporter, permease component 2 (GtsC) b1312 b4032
gtsD glucose ABC transporter, ATPase component (GtsD) b4035 b3450
kguD 2-keto-6-phosphogluconate reductase b3553 b2913
kguK 2-ketogluconokinase
kguT 2-ketogluconate transporter
manX glucose PTS, enzyme EIIAB b1817
manY glucose PTS, enzyme EIIC b1818
manZ glucose PTS, enzyme EIID b1819 b3140
MFS-glucose glucose transporter, MFS superfamily b2943 b2841
PAST-A proton-associated sugar transporter A
ptsG glucose PTS, enzyme IICB b1101 b0679
ptsG-crr glucose PTS, enzyme II (CBA components, PtsG) b0679 b1101
SemiSWEET Sugar transporter SemiSWEET
SSS-glucose Sodium/glucose cotransporter b3679
SWEET1 bidirectional sugar transporter SWEET1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory