Aligments for a candidate for MFS-glucose in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Aligments for a candidate for MFS-glucose in Escherichia coli BW25113

Align Galactose-proton symporter; Galactose transporter (characterized)
to candidate 16922 b2841 arabinose transporter (NCBI)

Query= SwissProt::P0AEP1
         (464 letters)



>FitnessBrowser__Keio:16922
          Length = 472

 Score =  595 bits (1534), Expect = e-174
 Identities = 296/456 (64%), Positives = 364/456 (79%), Gaps = 3/456 (0%)

Query: 9   RSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIADEFQITSHTQEWVVSSMMFGAAVG 68
           R  + M  FV   AA+AGLLFGLDIGVIAGALPFI D F +TS  QEWVVSSMM GAA+G
Sbjct: 16  RDTRRMNMFVSVAAAVAGLLFGLDIGVIAGALPFITDHFVLTSRLQEWVVSSMMLGAAIG 75

Query: 69  AVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAAPNVEVLILSRVLLGLAVGVASYTAPL 128
           A+ +GWLSF+LGRK SLM GAILFV GS+ SA A +VE+LI +RV+LG+AVG+ASYTAPL
Sbjct: 76  ALFNGWLSFRLGRKYSLMAGAILFVLGSIGSAFATSVEMLIAARVVLGIAVGIASYTAPL 135

Query: 129 YLSEIAPEKIRGSMISMYQLMITIGILGAYLSDTAFSYTGAWRWMLGVIIIPAILLLIGV 188
           YLSE+A E +RG MISMYQLM+T+GI+ A+LSDTAFSY+G WR MLGV+ +PA+LL+I V
Sbjct: 136 YLSEMASENVRGKMISMYQLMVTLGIVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILV 195

Query: 189 FFLPDSPRWFAAKRRFVDAERVLLRLRDTSAEAKRELDEIRESLQVKQSGWALFKENSNF 248
            FLP+SPRW A K R ++AE VL  LRDTS +A+ EL+EIRESL++KQ GWALFK N N 
Sbjct: 196 VFLPNSPRWLAEKGRHIEAEEVLRMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNV 255

Query: 249 RRAVFLGVLLQVMQQFTGMNVIMYYAPKIFELAGYTNTTEQMWGTVIVGLTNVLATFIAI 308
           RRAVFLG+LLQ MQQFTGMN+IMYYAP+IF++AG+T T +QM  T++VGLT + ATFIA+
Sbjct: 256 RRAVFLGMLLQAMQQFTGMNIIMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAV 315

Query: 309 GLVDRWGRKPTLTLGFLVMAAGMGVLG---TMMHIGIHSPSAQYFAIAMLLMFIVGFAMS 365
             VD+ GRKP L +GF VMA G  VLG        G  S    + ++ M +M I G+AMS
Sbjct: 316 FTVDKAGRKPALKIGFSVMALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMS 375

Query: 366 AGPLIWVLCSEIQPLKGRDFGITCSTATNWIANMIVGATFLTMLNTLGNANTFWVYAALN 425
           A P++W+LCSEIQPLK RDFGITCST TNW++NMI+GATFLT+L+++G A TFW+Y ALN
Sbjct: 376 AAPVVWILCSEIQPLKCRDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALN 435

Query: 426 VLFILLTLWLVPETKHVSLEHIERNLMKGRKLREIG 461
           + F+ +T WL+PETK+V+LEHIER LM G KLR IG
Sbjct: 436 IAFVGITFWLIPETKNVTLEHIERKLMAGEKLRNIG 471


Lambda     K      H
   0.327    0.140    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 779
Number of extensions: 37
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 464
Length of database: 472
Length adjustment: 33
Effective length of query: 431
Effective length of database: 439
Effective search space:   189209
Effective search space used:   189209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources