GapMind for catabolism of small carbon sources

 

Aligments for a candidate for bztB in Escherichia coli BW25113

Align glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized)
to candidate 1936976 b3269 predicted amino-acid transporter subunit (NCBI)

Query= CharProtDB::CH_011913
         (426 letters)



>lcl|FitnessBrowser__Keio:1936976 b3269 predicted amino-acid
           transporter subunit (NCBI)
          Length = 393

 Score =  270 bits (691), Expect = 4e-77
 Identities = 165/405 (40%), Positives = 231/405 (57%), Gaps = 33/405 (8%)

Query: 24  RSITIQIVVLLLFLAGLVWLLNNAYVNLEAKGKDFNFSFLWTRAGYDLAQTLIPYSNDDT 83
           R+   QI+ ++  +  + WL +N   NL  +G    F+FL   AG+ + Q LI Y   DT
Sbjct: 20  RAWLFQILAVVAVVGIVGWLFHNTVTNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDT 79

Query: 84  HFRALIEGLLNTLLVSVLGCILATILGTIIGVLRLSQNWLVARIMTVYVETFRNIPLLLW 143
           + R  I GLLNTLLVS L  + A++LG  IG+ RLS NWL+ ++ T+Y+E FRNIP LL 
Sbjct: 80  YGRVFIVGLLNTLLVSALCIVFASVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQ 139

Query: 144 ILLMGTILAETRPVPKDFRLTEAMKAAGEEPKASMWFFDSVAVTNRGTNLPAPAFDHSLG 203
           I      +    P P+     +A+ A           FD   ++NRG  +P+P       
Sbjct: 140 IFFWYFAVLRNLPGPR-----QAVSA-----------FDLAFLSNRGLYIPSP------- 176

Query: 204 VVDLGWNLPVSLNALAILAVMSASFWGWRRFMARAKAVQEATGTRPTTW-WPSLLILFAP 262
              LG      + A+ +  V+S   + +       K  Q  TG    TW   ++LI+  P
Sbjct: 177 --QLGDGFIAFILAVVMAIVLSVGLFRFN------KTYQIKTGQLRRTWPIAAVLIIGLP 228

Query: 263 ISAL-LYGLGFHLDYPQITKFDFTGGFQMLHSFTALLIALTLYTAAFIAEIVRAGIQAIS 321
           + A  L+G   H D P +  F+F GG  ++    AL +AL++YT+AFIAEI+RAGIQA+ 
Sbjct: 229 LLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVP 288

Query: 322 RGQTEAAYALGLRPGRTMSLVILPQALRVIVPPLISQFLNLTKNSSLAIAVSYMDLRGTL 381
            GQ EAA +LGL    T+  VI+PQALRVI+PPL SQ+LN+ KNSSLA A+ Y D+    
Sbjct: 289 YGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF 348

Query: 382 GGITLNQTGRELECMLLMMLIYLTISLTISSLMNLYNKSIKLKER 426
            G  LNQTG+ +E + + M +YL ISLTIS LMN+YN+ I + ER
Sbjct: 349 AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER 393


Lambda     K      H
   0.326    0.139    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 391
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 426
Length of database: 393
Length adjustment: 31
Effective length of query: 395
Effective length of database: 362
Effective search space:   142990
Effective search space used:   142990
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory