GapMind for catabolism of small carbon sources

 

Finding step gltJ for L-glutamate catabolism in Escherichia coli BW25113

4 candidates for gltJ: L-glutamate ABC transporter, permease component 1 (gltJ/aatQ)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi b0654 glutamate and aspartate transporter subunit (NCBI) Glutamate/aspartate import permease protein GltJ (characterized) 100% 100% 495.4 NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB 32% 120.9
lo b0653 glutamate and aspartate transporter subunit (NCBI) PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized) 31% 95% 120.9 Glutamate/aspartate import permease protein GltK 100% 431.0
lo b1918 predicted transporter subunit: membrane component of ABC superfamily (NCBI) PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized) 32% 87% 115.9 L-cystine transport system permease protein YecS 100% 433.7
lo b3269 predicted amino-acid transporter subunit (NCBI) Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) 39% 51% 103.2 ABC transporter for D-Alanine, permease component 2 63% 476.1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step gltJ

Or cluster all characterized gltJ proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory