Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate 14988 b0863 arginine transporter subunit (NCBI)
Query= TCDB::Q9HU31 (250 letters) >FitnessBrowser__Keio:14988 Length = 243 Score = 178 bits (452), Expect = 8e-50 Identities = 100/246 (40%), Positives = 149/246 (60%), Gaps = 11/246 (4%) Query: 5 KKILLAAAATLAFALDASAADKLRIGTEGAYPPFNGIDASGQAVGFDLDIGKALCAKMKT 64 KK+L+AA F+L A+AA+ +R TE +YPPF IDA+ Q VGFD+D+ +ALC ++ Sbjct: 2 KKVLIAALIA-GFSLSATAAETIRFATEASYPPFESIDANNQIVGFDVDLAQALCKEIDA 60 Query: 65 ECEVVTSDWDGIIPALNAKKFDFIVASMSITDERKQAVDFTDPYYTNKLQFVAPKSVDFK 124 C +D +IP+L ++ + ++A M IT ER++ V FT PYY N FV + Sbjct: 61 TCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTTPYYDNSALFVGQQGK--Y 118 Query: 125 TDKDSLKGKVIGAQRATIAGTWLEDNMADVVTIKLYDTQENAYLDLSSGRLDGVLADKFV 184 T D LKGK +G Q T ++ D ++ T+ YD+ +NA LDL +GR+DGV D V Sbjct: 119 TSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVP-YDSYQNAKLDLQNGRIDGVFGDTAV 177 Query: 185 QYDWLKSDAGKEFEFKGEPVFDND----KIGIAVRKGD-PLREKLNAALKEIVADGTYKK 239 +WLK + + G+ V D D +GIAVR+G+ L++KLN AL+++ DGTY+ Sbjct: 178 VTEWLKDN--PKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQKLNTALEKVKKDGTYET 235 Query: 240 INDKYF 245 I +K+F Sbjct: 236 IYNKWF 241 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 178 Number of extensions: 10 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 250 Length of database: 243 Length adjustment: 24 Effective length of query: 226 Effective length of database: 219 Effective search space: 49494 Effective search space used: 49494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory