Alignments for a candidate for lacL in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacL in Escherichia coli BW25113

Align LVIS_2259 + LVIS_2258 (EC 3.2.1.23) (characterized)
to candidate 1936924 b3076 cryptic beta-D-galactosidase, alpha subunit (NCBI)

Query= CAZy::ABJ65308.1
         (628 letters)



>FitnessBrowser__Keio:1936924
          Length = 1030

 Score =  345 bits (884), Expect = 7e-99
 Identities = 212/618 (34%), Positives = 329/618 (53%), Gaps = 51/618 (8%)

Query: 17  NQLPAHSDHRGYASVEEATA---QHSSLVQSLDGTWQFAFAPDPVHRFEGFYQPDYDRSA 73
           N+L   +    Y SV +A     + SSL   L G W F F   P+   E F       + 
Sbjct: 13  NRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVPEAFTSELM--AD 70

Query: 74  FDRLTVPGHIELAGYGQIQYINTAYPWEGHHYRRPAYSMGADQPEKGMFSTDPQNTVGAY 133
           +  +TVP   ++ G+G++QY +  +P+              D P          N  GAY
Sbjct: 71  WGHITVPAMWQMEGHGKLQYTDEGFPFP------------IDVP-----FVPSDNPTGAY 113

Query: 134 VKHFTLNPALANQRVSIEFDGVEQAMFLWLNGQFVGYAEDSFSRSEFDLTPYLQAGQNLL 193
            + FTL+     ++  I+FDGVE    +++NGQ+VG+++ S   +EFD++  ++ G NLL
Sbjct: 114 QRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVKTGDNLL 173

Query: 194 AVEVFKHSTAAFLEDQDMFRFSGIFRSVRLVAKPELHVEDLTIRAGLDDAFQTGDLKVRL 253
            V V + + + ++EDQDM+  +GIFR V LV K   H+ D T+R   D+A+    L   +
Sbjct: 174 CVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEV 233

Query: 254 QLTAASQLSGTATAQLLTADGQEVW---ATEQPAASTLDLAA---AIDHVHLWDHHDPYL 307
            L   +      T +    DG+ V    A +  A   L  A+    ++    W    PYL
Sbjct: 234 VLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASFAFTVEQPQQWSAESPYL 293

Query: 308 YQLRITLKDVAGQVVEVVPYPVGFRRIELKDKVMCLNGQRLILNGVNRHEWDAHRGRAVT 367
           Y L +TLKD  G V+EVVP  VGFR I+++D +  +N + ++L+GVNRH+ D  +GRAV 
Sbjct: 294 YHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVG 353

Query: 368 MADMTQDLQTFHDNHINAVRTCHYPDQDAWYYLCDQQGIYMMAENNLETHGTWQKMGAVE 427
           M  + +DLQ    ++IN+VRT HYP+   +Y LCD  G+++MAE ++E+HG +  +G + 
Sbjct: 354 MDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHG-FANVGDIS 412

Query: 428 PSYNVPGSLPQWQLAVLDRAKSNYEMFKNHPAVLFWSLGNESYAGDNIAAMDAFYHHA-- 485
              +     PQW+   ++R   +    KNHP+++ WSLGNES  G NI AM   YH A  
Sbjct: 413 RITDD----PQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAM---YHAAKA 465

Query: 486 -DPTRLTHYEGVCRNRVYEDR---ISDMESMMYDPPRAIEDYLKNDPQKPFVNCEYMHDM 541
            D TRL HYE        EDR   + D+ S MY     + ++ +    KP + CEY H M
Sbjct: 466 LDDTRLVHYE--------EDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPRIICEYAHAM 517

Query: 542 GNSLGGMASYDALIDQYPMYQGGFIWDFIDQALWVKDEVTGQPVLRYGGDFDDRHSDYEF 601
           GN  GG+  Y  +  ++   QG ++W++ D  +  +D+  G    ++GGD+ D  ++Y F
Sbjct: 518 GNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDD-HGNVWYKFGGDYGDYPNNYNF 576

Query: 602 SGDGLLFADRTPKPALQE 619
             DGL+++D+TP P L+E
Sbjct: 577 CLDGLIYSDQTPGPGLKE 594


Lambda     K      H
   0.320    0.136    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1888
Number of extensions: 103
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 628
Length of database: 1030
Length adjustment: 41
Effective length of query: 587
Effective length of database: 989
Effective search space:   580543
Effective search space used:   580543
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources