Alignments for a candidate for aacS in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Escherichia coli BW25113

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate 15820 b1701 short chain acyl-CoA synthetase, anaerobic (RefSeq)

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>FitnessBrowser__Keio:15820
          Length = 548

 Score =  258 bits (660), Expect = 3e-73
 Identities = 174/545 (31%), Positives = 278/545 (51%), Gaps = 30/545 (5%)

Query: 28  EQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLGMGLTPGDRVGIW 87
           + ++  ++       P++ A+V  H G  YTY+ L   A  LA+ +L  G+  GDR+   
Sbjct: 23  DASLADYWQQTARAMPDKIAVVDNH-GASYTYSALDHAASCLANWMLAKGIESGDRIAFQ 81

Query: 88  SHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTSDYLGML 147
                E+ ++ LA  ++G V V + P++R AE+ + LNK   K+  +   FK +  + ++
Sbjct: 82  LPGWCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLI 141

Query: 148 RELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIARGNAADPRLA 207
             L  +             LPQL+ +V +D  A   +     L  +++IA   +    + 
Sbjct: 142 LPLQNQ-------------LPQLQQIVGVDKLAPATSS----LSLSQIIADNTSLTTAIT 184

Query: 208 QVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKLTPADRLCIPVPLY 267
                L A     + FTSGT G PKG  LTH NIL +       + LT  D   +P PL 
Sbjct: 185 THGDELAA-----VLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLG 239

Query: 268 HCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAELDHPRFA 327
           H  G + G  A F  GA  V   D F P   L  ++ +RCT + G        L+     
Sbjct: 240 HATGFLHGVTAPFLIGARSVLL-DIFTPDACLALLEQQRCTCMLGATPFVYDLLNVLEKQ 298

Query: 328 EFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTDTPLSKRV 387
             +LS LR  +  G+  P +V  R  +Q  ++ +++ YG TE+SP +  +  D PLS+ +
Sbjct: 299 PADLSALRFFLCGGTTIPKKVA-RECQQRGIKLLSV-YGSTESSPHAVVN-LDDPLSRFM 355

Query: 388 STVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTREAIDEGGWMH 447
            T G     +E+K+VD D    +P G  GE  ++G +V  GY+ +   T  A+DE GW +
Sbjct: 356 HTDGYAAAGVEIKVVD-DARKTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYY 414

Query: 448 TGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQVVGVPDQKYGE 507
           +GDL  MD  GY+ I GR KD+++RGGENI  RE+E+ L +HP++ D  VV + D++ GE
Sbjct: 415 SGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMSDERLGE 474

Query: 508 ELCAWIIAKP--GTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKIQKFKIRDE 565
             CA+++ K    +   E+ +  F + ++A YK P +I  +   P T +GKIQKF +R +
Sbjct: 475 RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTTSGKIQKFLLRKD 534

Query: 566 MKDQL 570
           +  +L
Sbjct: 535 IMRRL 539


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 700
Number of extensions: 33
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 548
Length adjustment: 36
Effective length of query: 542
Effective length of database: 512
Effective search space:   277504
Effective search space used:   277504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources