GapMind for catabolism of small carbon sources

 

Aligments for a candidate for liuA in Escherichia coli BW25113

Align isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (characterized)
to candidate 15814 b1695 putative oxidoreductase (VIMSS)

Query= BRENDA::Q75N94
         (430 letters)



>lcl|FitnessBrowser__Keio:15814 b1695 putative oxidoreductase
           (VIMSS)
          Length = 383

 Score =  166 bits (421), Expect = 9e-46
 Identities = 123/386 (31%), Positives = 183/386 (47%), Gaps = 13/386 (3%)

Query: 46  TEDELLELRERVQEFTRREITEEVAAKTDAQNEFPAEMWKKLGDAGFLGITANEDYGGLG 105
           TE++ L L   ++E       EE     D    +P E  + L D G   +   E++GG+ 
Sbjct: 6   TEEQELLLAS-IRELITTNFPEEYFRTCDQNGTYPREFMRALADNGISMLGVPEEFGGIP 64

Query: 106 MGYQAHCIVMEELSRASGSIALSYAAHSQLCVNQLSLNGSPEQKERFLPGLL-SGDKIGA 164
             Y    + + E+S+     A ++   +  C++ +   GS EQ  +     L +GD   A
Sbjct: 65  ADYVTQMLALMEVSKCG---APAFLITNGQCIHSMRRFGSAEQLRKTAESTLETGDPAYA 121

Query: 165 LAMSEHSAGSDVVSMKTTAKEVDGGYVLNGTKMWITNGPDADFIVVYAKT-EPQKGSKGI 223
           LA++E  AGSD  S  TT    +G   +NG K +IT   +  +++V A+  +P+   K  
Sbjct: 122 LALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAKEYPYMLVLARDPQPKDPKKAF 181

Query: 224 TAFVVEKTFDGFSCARKLDKLGMRGSNTGELIFEDVFVPKENVLGEVNRGVKVLMEGLDL 283
           T + V+ +  G      L K+G    +T E+  ++V V + +++GE   G   +M   ++
Sbjct: 182 TLWWVDSSKPGIKI-NPLHKIGWHMLSTCEVYLDNVEVEESDMVGEEGMGFLNVMYNFEM 240

Query: 284 ERLVLSAGPLGIMQAALDLVLPYTHVRKQFGTPIAHNQLIQGKLADMYTKLQASRAYTYS 343
           ERL+ +A   G  + A +    Y + R  FG PI HNQ+IQ KLA M  K+   R     
Sbjct: 241 ERLINAARSTGFAECAFEDAARYANQRIAFGKPIGHNQMIQEKLALMAIKIDNMRNMVLK 300

Query: 344 TARHIDNSASLSEVSIRTQDCAGAILYAAERATECALDAIQLMGGNGYINELPAGRLLRD 403
            A   D   SL     RT   A A LY A  A E   DAIQ+MGG GY +E    R  RD
Sbjct: 301 VAWQADQHQSL-----RT-SAALAKLYCARTAMEVIDDAIQIMGGLGYTDEARVSRFWRD 354

Query: 404 AKLYEIGAGTSEIRRMVIGRAFNKEY 429
            +   IG GT EI   V GR   K+Y
Sbjct: 355 VRCERIGGGTDEIMIYVAGRQILKDY 380


Lambda     K      H
   0.317    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 385
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 383
Length adjustment: 31
Effective length of query: 399
Effective length of database: 352
Effective search space:   140448
Effective search space used:   140448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory