GapMind for catabolism of small carbon sources

 

Finding step livG for L-leucine catabolism in Escherichia coli BW25113

5 candidates for livG: L-leucine ABC transporter, ATPase component 2 (LivG/BraF)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi b3455 leucine/isoleucine/valine transporter subunit (NCBI) High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 100% 100% 506.5 Putative branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 44% 219.2
lo b3201 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 34% 100% 151.8 lipopolysaccharide ABC transporter, ATP-binding protein LptB; EC 3.6.3.- 100% 471.9
lo b1917 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 32% 98% 137.5 L-cystine transport system ATP-binding protein YecC; EC 7.4.2.- 100% 481.1
lo b3749 fused D-ribose transporter subunits of ABC superfamily: ATP-binding components (NCBI) ABC transporter ATP-binding protein (characterized, see rationale) 33% 96% 134.8 ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 100% 971.8
lo b4087 fused D-allose transporter subunits of ABC superfamily: ATP-binding components (NCBI) High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 32% 100% 133.3 D-allose import ATP-binding protein AlsA; EC 7.5.2.8 100% 1004.2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step livG

Or cluster all characterized livG proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory