GapMind for catabolism of small carbon sources

 

Aligments for a candidate for frcC in Escherichia coli BW25113

Align Fructose import permease protein FrcC (characterized)
to candidate 16257 b2148 beta-methylgalactoside transporter inner membrane component (NCBI)

Query= SwissProt::Q9F9B1
         (360 letters)



>lcl|FitnessBrowser__Keio:16257 b2148 beta-methylgalactoside
           transporter inner membrane component (NCBI)
          Length = 336

 Score =  175 bits (443), Expect = 2e-48
 Identities = 106/313 (33%), Positives = 169/313 (53%), Gaps = 14/313 (4%)

Query: 52  LIVLVLSLIAFGVILGGKFFSAFTMTLILQQVAIVGIVGAAQTLVILTAGIDLSVGAIMV 111
           + V++L L+A  +     F S   ++ IL Q ++  I+      +I+T G DLS G  + 
Sbjct: 18  IYVVLLVLLAIIIFQDPTFLSLLNLSNILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVG 77

Query: 112 LSSVIMGQ-----------FTFRYGFPPALSVICGLGVGALCGYINGTLVARMKLPPFIV 160
           L++V+              F      P AL ++    +GA+ G ING ++A + + PFI 
Sbjct: 78  LAAVVAATLLQSMDNANKVFPEMATMPIALVILIVCAIGAVIGLINGLIIAYLNVTPFIT 137

Query: 161 TLGMWQIVLASNFLYSANETIRAQDISANASILQFFGQNF-RIGNAVFTYGVVVMVLLVC 219
           TLG   IV   N LY   + + A  IS   S    F Q F  +G+   +Y     ++ V 
Sbjct: 138 TLGTMIIVYGINSLYY--DFVGASPISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVA 195

Query: 220 LLWYVLNRTAWGRYVYAVGDDPEAAKLAGVNVTRMLISIYTLSGLICALAGWALIGRIGS 279
            +W + N+T +G+ ++A+G +PEAAK++GVNV   L+ IY LSG+  A  G    GRIGS
Sbjct: 196 FVWVLWNKTRFGKNIFAIGGNPEAAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGS 255

Query: 280 VSPTAGQFANIESITAVVIGGISLFGGRGSIMGMLFGALIVGVFSLGLRLMGTDPQWTYL 339
            +   G    +++I A V+GG+S  GG G+++G++ G +I  V + GL  +G +P W Y+
Sbjct: 256 ATNNLGFMYELDAIAACVVGGVSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYI 315

Query: 340 LIGLLIIIAVAID 352
           + G +II AVA+D
Sbjct: 316 IKGAIIIFAVALD 328


Lambda     K      H
   0.327    0.141    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 319
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 360
Length of database: 336
Length adjustment: 29
Effective length of query: 331
Effective length of database: 307
Effective search space:   101617
Effective search space used:   101617
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory