Alignments for a candidate for manMFS in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for manMFS in Escherichia coli BW25113

Align D-mannose and D-mannitol transporter (characterized)
to candidate 17750 b3691 D-galactonate transport (VIMSS)

Query= reanno::pseudo5_N2C3_1:AO356_28540
         (430 letters)



>FitnessBrowser__Keio:17750
          Length = 430

 Score =  202 bits (514), Expect = 2e-56
 Identities = 129/413 (31%), Positives = 211/413 (51%), Gaps = 22/413 (5%)

Query: 3   GQGRSLIIIMLFLAGVINYLDRSALSVAAPFIQKDYGLSTGEMGMIFSSFFVGYAAFNFI 62
           G+ R L ++M+F+  VI Y+DR+ L+VA+  IQ+++G++  EMG +FS+F   Y      
Sbjct: 11  GRRRYLTLVMIFITVVICYVDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIP 70

Query: 63  GGWAADRYGAKTTLLLAMVLWSLFSGLTVLTVGFASLVLIRILFGMGEGPLSVTTSKMVN 122
           GGW  DR G++ T  +A+  WS+ +       G  SL+ +R + G+ E P   T ++MV 
Sbjct: 71  GGWFLDRVGSRVTYFIAIFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVT 130

Query: 123 NWYTPKRRARAIGASMSGTPLGGAISGPVVGFIAVTYGWKISFIIIMLIGLVWAAVWFKF 182
           +W+    RA A+G   SG  +G A   P++ +I     W   FI+   IG++W+ +WFK 
Sbjct: 131 SWFPEHERASAVGFYTSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKV 190

Query: 183 VKERPEGEGAE----DILRAEG---QGEL-----AAQPV----FPLRFYLKQPTVLFTSL 226
            +     +G      D +R  G    G+      A QP+    + L F+ K   ++   L
Sbjct: 191 YQPPRLTKGISKAELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRK---LIGVYL 247

Query: 227 AFFSYNYTLFFFLTWFPSYLTMAHGLNVKDMSIATVIPWVLGFLGLALGGFISDFVFKKT 286
             F+   TL+FFLTWFP+YLT   G+        T +P++  F+G+ L G+++D + +K 
Sbjct: 248 GQFAVASTLWFFLTWFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKG 307

Query: 287 GRMMFSRKVVLVTCLLACAVCIACAGMVTTLYPAVILVALAVFFLYLTGAIYWAIIQDTV 346
             + F+RK  ++ C L  + CI  A         + L+ALA FF     +I W+++    
Sbjct: 308 FSLGFARKTPII-CGLLISTCIMGANYTNDPMMIMCLMALA-FFGNGFASITWSLVSSLA 365

Query: 347 PAARVGGVSGFMHFLANTSGIVGPTLTGFLVQFTGSFTSAFLLAGLLTVIGAV 399
           P   +G   G  +F     GI  P + G+L Q  G F  A +    + +IGA+
Sbjct: 366 PMRLIGLTGGVFNFAGGLGGITVPLVVGYLAQGYG-FAPALVYISAVALIGAL 417



 Score = 33.9 bits (76), Expect = 1e-05
 Identities = 33/161 (20%), Positives = 66/161 (40%), Gaps = 13/161 (8%)

Query: 33  FIQKDYGLSTGEMGMIFSSFFVGYAAFNFIGGWAADR---------YGAKTTLLLAMVLW 83
           ++ ++ G++  + G + +  F+       + GW AD          +  KT ++  +++ 
Sbjct: 266 YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLLIS 325

Query: 84  SLFSGLTVLTVGFASLVLIRILFGMGEGPLSVTTSKMVNNWYTPKRRARAIGASMS-GTP 142
           +   G          + L+ + F  G G  S+T S + +    P R     G   +    
Sbjct: 326 TCIMGANYTNDPMMIMCLMALAF-FGNGFASITWSLVSS--LAPMRLIGLTGGVFNFAGG 382

Query: 143 LGGAISGPVVGFIAVTYGWKISFIIIMLIGLVWAAVWFKFV 183
           LGG     VVG++A  YG+  + + I  + L+ A  +   V
Sbjct: 383 LGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLV 423


Lambda     K      H
   0.329    0.142    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 604
Number of extensions: 35
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 430
Length of database: 430
Length adjustment: 32
Effective length of query: 398
Effective length of database: 398
Effective search space:   158404
Effective search space used:   158404
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources