myo-inositol catabolism in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

myo-inositol catabolism in Escherichia coli BW25113

Best path

iolT, iolG, iolM, iolN, iolO, uxaE, uxuB, uxuA, kdgK, eda

Also see fitness data for the top candidates

Rules

Overview: Myo-inositol degradation in GapMind is based on MetaCyc pathways myo-inositol degradation I via inosose dehydratase (link) and pathway II inosose dehydrogenase (link).

all:

myo-inositol-transport, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA and tpi
or myo-inositol-transport, iolG, iolM, iolN, iolO, uxaE, uxuB, uxuA, kdgK and eda
Comment: Both pathways begin with the 2-dehydrogenase (iolG) forming scyllo-inosose. In pathway I, inosose dehydratase (iolE) forms 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione, followed by a ring-cleaving hydrolase to 5-deoxy-D-glucuronate, isomerization to 5-dehydro-2-deoxy-D-gluconate, phosphorylation, and cleavage by an aldolase to 3-oxopropionate (malonate semialdehyde) and glycerone phosphate; the 3-oxopropionate is oxidized to acetyl-CoA while the glycerone phosphate is converted by triose-phosphate isomerase to glyceraldehyde 3-phosphate. In pathway II, a dehydrogenase forms 3-dehydro scyllo-inosose (also known as 2,4-diketo-inositol), a hydratase forms 5-dehydro-L-gluconate, an epimerase forms D-tagaturonate, another forms to D-fructonate, a reductase forms D-mannonate, a dehydratase forms 2-keto-3-deoxygluconate, a kinase forms 2-keto-3-deoxy-gluconate 6-phosphate, and an aldolase forms glyceraldehyde-3-phosphate and pyruvate.

myo-inositol-transport:

PGA1_c07300, PGA1_c07310 and PGA1_c07320
or iatP, iatA and ibpA
or PS417_11885, PS417_11890 and PS417_11895
or iolT
or SMIT1
or HMIT
or iolF
Comment: Transporters were identified using: query: transporter:myo-inositol:myoinositol:m-inositol:inositol

29 steps (18 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
iolT	myo-inositol:H+ symporter	b4031	b2943
iolG	myo-inositol 2-dehydrogenase
iolM	2-inosose 4-dehydrogenase	b2545
iolN	2,4-diketo-inositol hydratase
iolO	5-dehydro-L-gluconate epimerase	b1314
uxaE	D-tagaturonate epimerase
uxuB	D-mannonate dehydrogenase	b4323	b2172
uxuA	D-mannonate dehydratase	b4322	b1581
kdgK	2-keto-3-deoxygluconate kinase	b3526
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	b1850	b4477
Alternative steps:
HMIT	myo-inositol:H+ symporter	b2943	b2841
iatA	myo-inositol ABC transporter, ATPase component IatA	b3749	b1900
iatP	myo-inositol ABC transporter, permease component IatP	b3750	b4086
ibpA	myo-inositol ABC transporter, substrate-binding component IbpA	b3751	b4088
iolB	5-deoxy-D-glucuronate isomerase
iolC	5-dehydro-2-deoxy-D-gluconate kinase
iolD	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
iolE	scyllo-inosose 2-dehydratase
iolF	myo-inositol:H+ symporter
iolJ	5-dehydro-2-deoxyphosphogluconate aldolase	b3137	b2096
mmsA	malonate-semialdehyde dehydrogenase	b2661	b0312
PGA1_c07300	myo-inositol ABC transport, substrate-binding component
PGA1_c07310	myo-inositol ABC transporter, permease component
PGA1_c07320	myo-inositol ABC transporter, ATPase component	b4087	b3749
PS417_11885	myo-inositol ABC transporter, substrate-binding component	b2150
PS417_11890	myo-inositol ABC transporter, ATPase component	b3749	b2149
PS417_11895	myo-inositol ABC transporter, permease component	b3750	b2148
SMIT1	myo-inositol:Na+ symporter
tpi	triose-phosphate isomerase	b3919	b2926

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources