Alignments for a candidate for iatA in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for iatA in Escherichia coli BW25113

Align Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized)
to candidate 16258 b2149 fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI)

Query= TCDB::B8H229
         (515 letters)



>FitnessBrowser__Keio:16258
          Length = 506

 Score =  360 bits (924), Expect = e-104
 Identities = 189/493 (38%), Positives = 310/493 (62%), Gaps = 6/493 (1%)

Query: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTF 62
           LL++S ++KSFPGV+ALD V+L V    +HAL+GENGAGKSTL+K L   +  D+GT+ F
Sbjct: 13  LLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTILF 72

Query: 63  AGQVLDPRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLRADAQ 122
            G+ +D   A    +  GI+ ++QE NL  + SV +NM+LGR P +   VD  ++  + +
Sbjct: 73  QGKEIDFHSAKEALEN-GISMVHQELNLVLQRSVMDNMWLGRYPTKGMFVDQDKMYRETK 131

Query: 123 ALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAI 182
           A+ ++L + ++P A V  L+V++ QM+EIAKA + NA+++IMDEPT++L+ +EV+ L  I
Sbjct: 132 AIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHLFTI 191

Query: 183 IAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRLMVGRHVE 242
           I  LK R   ++Y+SH++ E+  +CD  TV+RDG+++A+  +A + +  ++ +MVGR + 
Sbjct: 192 IRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLN 251

Query: 243 FERRKRRRPPGAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLA 302
                +   PG V+L+V  +T     L  P  +R VSF    GEI+G+AGLVGA RTD+ 
Sbjct: 252 QRFPDKENKPGEVILEVRNLT----SLRQPS-IRDVSFDLHKGEILGIAGLVGAKRTDIV 306

Query: 303 RLIFGADPIAAGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLSLPS 362
             +FG    +AG + +  K +   +  +AI  G  LV E+R+  G +    I  N  + +
Sbjct: 307 ETLFGIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYLDIGFNSLISN 366

Query: 363 LKALSALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTPK 422
           ++        +D    +   +     +R+K     T IG LSGGNQQKV++GR +   P+
Sbjct: 367 IRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPE 426

Query: 423 VLIVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVA 482
           +L++DEPTRGID+GAK E++Q++++LA  G  +++ISSE+ E++ ++DRI+V   G++  
Sbjct: 427 ILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSG 486

Query: 483 DLDAQTATEEGLM 495
            +D +T T+  ++
Sbjct: 487 IVDTKTTTQNEIL 499



 Score = 80.9 bits (198), Expect = 1e-19
 Identities = 63/259 (24%), Positives = 116/259 (44%), Gaps = 10/259 (3%)

Query: 253 GAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFGADPIA 312
           G  +L++ G+  + P + A   L  V+   R   I  L G  GAG++ L + +FG     
Sbjct: 10  GEYLLEMSGINKSFPGVKA---LDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKD 66

Query: 313 AGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLSLPSLKALSALGQW 372
           +G +L   K +   S ++A++ GI +V    ++    L  S+  N+ L         G +
Sbjct: 67  SGTILFQGKEIDFHSAKEALENGISMV---HQELNLVLQRSVMDNMWLGRYPTK---GMF 120

Query: 373 VDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTPKVLIVDEPTRG 432
           VD+       +    +L I + D    +G LS    Q + + +A +   K++I+DEPT  
Sbjct: 121 VDQDKMYRETKAIFDELDIDI-DPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSS 179

Query: 433 IDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVADLDAQTATEE 492
           +       +  ++  L + G  +V IS ++ E+  + D + V R+G  +A       T +
Sbjct: 180 LTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMD 239

Query: 493 GLMAYMATGTDRVAAPDME 511
            ++A M   +     PD E
Sbjct: 240 KIIAMMVGRSLNQRFPDKE 258



 Score = 61.2 bits (147), Expect = 8e-14
 Identities = 47/214 (21%), Positives = 98/214 (45%), Gaps = 8/214 (3%)

Query: 29  GEVHALLGENGAGKSTLIKILSAAHAADAGTVTFAGQVLDPRDAPLRRQQLGIATIYQE- 87
           GE+  + G  GA ++ +++ L       AGT+T  G+ ++  +A       G A + +E 
Sbjct: 289 GEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANEAINH-GFALVTEER 347

Query: 88  --FNLFPELSVAENMYLGR---EPRRLGLVDWSRLRADAQALLNDLGLPL-NPDAPVRGL 141
               ++  L +  N  +        ++GL+D SR+++D Q +++ + +        +  L
Sbjct: 348 RSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSL 407

Query: 142 TVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAIIAGLKARSVSVIYVSHRLG 201
           +   QQ V I + +     ++++DEPT  +       ++ +IA L  +   +I +S  + 
Sbjct: 408 SGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMP 467

Query: 202 EVKAMCDRYTVMRDGRFVASGDVADVEVADMVRL 235
           E+  + DR  VM +G      D       +++RL
Sbjct: 468 ELLGITDRILVMSNGLVSGIVDTKTTTQNEILRL 501


Lambda     K      H
   0.320    0.136    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 558
Number of extensions: 26
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 515
Length of database: 506
Length adjustment: 35
Effective length of query: 480
Effective length of database: 471
Effective search space:   226080
Effective search space used:   226080
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources