GapMind for catabolism of small carbon sources

 

Aligments for a candidate for badK in Escherichia coli BW25113

Align BadK (characterized)
to candidate 14182 b0036 crotonobetainyl CoA hydratase (RefSeq)

Query= metacyc::MONOMER-943
         (258 letters)



>lcl|FitnessBrowser__Keio:14182 b0036 crotonobetainyl CoA hydratase
           (RefSeq)
          Length = 261

 Score =  129 bits (325), Expect = 5e-35
 Identities = 96/264 (36%), Positives = 135/264 (51%), Gaps = 9/264 (3%)

Query: 1   MSSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAG-NTR 59
           MS +  LT   G +  ITL+RP   NA++      +G   L F  D  +   +I G   +
Sbjct: 1   MSESLHLTRN-GSILEITLDRPKA-NAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEK 58

Query: 60  AFAAGADIASMAAWSYSDV-YGSNFITRNWETIRQIRKPVLAAVAGLAYGGGCELALACD 118
            F+AG D+ + A     D  +G        E I  + KPV+AAV G A+GGG ELALA D
Sbjct: 59  FFSAGWDLKAAAEGEAPDADFGPGGFAGLTE-IFNLDKPVIAAVNGYAFGGGFELALAAD 117

Query: 119 IVIAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCLSARPLNAEEADRYGLVS 178
            ++   +A FALPE KLG++P +GG  RLP+ +  A   +M ++ R + AEEA R+G+V+
Sbjct: 118 FIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGIVN 177

Query: 179 RVVDDDRLRDETVALATTIAAFSAPALMALKESLNRAFESTLAEGILFERRELHARFASA 238
           RVV    L D    LA  +   +  A+ ALKE      E  + E   + R  +   + S 
Sbjct: 178 RVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSV 237

Query: 239 ----DAREGIQAFLEKRAPCFSHR 258
               DA EG  AF EKR P +  R
Sbjct: 238 LHSEDAIEGPLAFAEKRDPVWKGR 261


Lambda     K      H
   0.321    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 106
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 258
Length of database: 261
Length adjustment: 24
Effective length of query: 234
Effective length of database: 237
Effective search space:    55458
Effective search space used:    55458
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory