Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate 14452 b0314 choline transporter of high affinity (NCBI)
Query= SwissProt::G3XCN6 (706 letters) >FitnessBrowser__Keio:14452 Length = 677 Score = 489 bits (1260), Expect = e-142 Identities = 258/660 (39%), Positives = 400/660 (60%), Gaps = 18/660 (2%) Query: 46 HQVQRFKVNLPVFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSG-FGWLYLLSVAV 104 H ++ K+N VF S +I LF + I + +++ G +S FGW YLL+ + Sbjct: 6 HSREKDKINPVVFYTSAGLILLF-SLTTILFRDFSALWIGRTLDWVSKTFGWYYLLAATL 64 Query: 105 FLFSMLFLAFSRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASP 164 ++ ++ +A SR+G +KLGP+ S+PEF LSW AMLFAAG+GI LM+F+V EP+T + P Sbjct: 65 YIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQP 124 Query: 165 PEAEPLTIAAQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKE 224 PE TI A R+AM T FH+G+ W++Y+++G++L YF YRYNLPLT+RS LYP+ + Sbjct: 125 PEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYPIFGK 184 Query: 225 GIHGPIGHVVDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAI 284 I+GPIGH VDI A+ GT+FG+AT+LG G++Q+N GL+ L IP S+ + L+ + I Sbjct: 185 RINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIALSVII 244 Query: 285 ATISVVTGVEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTF 344 ATISV +GV+KG+R+LSE N+ LA+ L+LFVL +G T L+ V N+G Y++ + T Sbjct: 245 ATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFMGMTL 304 Query: 345 NIYAYE-PRPWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFL 403 N +A++ P W+++WTLF+WAWW++WSPFVG+F+ARISRGRT+R+FV L +P FT L Sbjct: 305 NSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFTFTLL 364 Query: 404 WMTVFGNTAIYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVT 463 W++VFGN+A+Y + A + + P ++++A + +F+VT Sbjct: 365 WLSVFGNSALY-EIIHGGAAFAEEAMVHPERGFYSLLAQYPAFTFSASVATITGLLFYVT 423 Query: 464 SSDSGSLVIDTIAS---GGETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTAL 520 S+DSG+LV+ S + P R+FW G++ +L T G++ALQ+ T+ L Sbjct: 424 SADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVIMGL 483 Query: 521 PFSLVMLILVWSLFVGMRA-DLARTQSPGSLGPRAYPASG-VPWQRRLAMTLSTPDRRAV 578 PFS V+ ++ L+ ++ D R + PR + W++RL+ ++ P R Sbjct: 484 PFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYT 543 Query: 579 EKFLQASVLPALEAVARELTRR-------SRPASVGRDAETGALTLTVPAEGHRDFVYGV 631 ++ ++ PA+E VA+EL R S P G+ + G L L V ++FVY + Sbjct: 544 KQMMETVCYPAMEEVAQELRLRGAYVELKSLPPEEGQ--QLGHLDLLVHMGEEQNFVYQI 601 Query: 632 QMSEHKLPAFTAYDATVADVRYEARTFFSDGSRGYDIMGMADNQIINDVLFQFERYTGFV 691 ++ +P FT + Y TF +GS+G D+M + Q+I D+L Q+ER+ F+ Sbjct: 602 WPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFI 661 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1262 Number of extensions: 68 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 677 Length adjustment: 39 Effective length of query: 667 Effective length of database: 638 Effective search space: 425546 Effective search space used: 425546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory