GapMind for catabolism of small carbon sources

 

Aligments for a candidate for davD in Escherichia coli BW25113

Align Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized)
to candidate 14450 b0312 betaine aldehyde dehydrogenase, NAD-dependent (NCBI)

Query= reanno::pseudo13_GW456_L13:PfGW456L13_495
         (480 letters)



>lcl|FitnessBrowser__Keio:14450 b0312 betaine aldehyde
           dehydrogenase, NAD-dependent (NCBI)
          Length = 490

 Score =  311 bits (796), Expect = 4e-89
 Identities = 169/477 (35%), Positives = 272/477 (57%), Gaps = 9/477 (1%)

Query: 6   TQLFRQQAFIDGAWVDADNGQTIKVNNPATGEILGTVPKMGAAETRRAIEAADKALPAWR 65
           +++  QQ +I G +  A +G+T +  NPA G +L TV   G  +  RA+++A +    W 
Sbjct: 2   SRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGREDVDRAVKSAQQGQKIWA 61

Query: 66  ALTAKERATKLRRWYELIIENQDDLARLMTLEQGKPLAEAKG-EIVYAASFIEWFAEEAK 124
           ++TA ER+  LRR  +++ E  D+LA+L TL+ GK  +E    +IV  A  +E++A    
Sbjct: 62  SMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETSTVDIVTGADVLEYYAGLIP 121

Query: 125 RIYGDVIPGHQPDKRLIVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVLKPASQ 184
            + G  IP  +    +   ++P+GV A I  WN+P  +   K+ PALAAG  M+ KP+  
Sbjct: 122 ALEGSQIPLRETSF-VYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEV 180

Query: 185 TPFSAFALAELAQRAGIPAGVFSVVSGSAGDIGSELTSNPIVRKLSFTGSTEIGRQLM-S 243
           TP +A  LAE+   AG+P GVF+V+ G   + G  LT +P + K+SFTG    G+++M +
Sbjct: 181 TPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTEHPGIAKVSFTGGVASGKKVMAN 240

Query: 244 ECAKDIKKVSLELGGNAPFIVFDDADLDKAVEGAIISKYRNNGQTCVCANRLYIQDGVYD 303
             A  +K+V++ELGG +P IVFDDADLD A + A+++ + ++GQ C    R+++      
Sbjct: 241 SAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMMANFFSSGQVCTNGTRVFVPAKCKA 300

Query: 304 AFAEKLKVAVAKLKIGNGLEAGTTTGPLIDEKAVAKVQEHIADALSKGATVLAGGKPMEG 363
           AF +K+   V +++ G+  +  T  GPL+       V  +IA    +GA VL GG  ++G
Sbjct: 301 AFEQKILARVERIRAGDVFDPQTNFGPLVSFPHRDNVLRYIAKGKEEGARVLCGGDVLKG 360

Query: 364 NFFE------PTILTNVPNNAAVAKEETFGPLAPLFRFKDEADVIAMSNDTEFGLASYFY 417
           + F+      PT+ T+  ++  + +EE FGP+  +  ++ E +VI  +NDT++GLA+   
Sbjct: 361 DGFDNGAWVAPTVFTDCSDDMTIVREEIFGPVMSILTYESEDEVIRRANDTDYGLAAGIV 420

Query: 418 ARDLGRVFRVAEALEYGMVGVNTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIK 474
             DL R  RV   LE G+  +NT   S    P GG K SG+GRE     ++ Y ++K
Sbjct: 421 TADLNRAHRVIHQLEAGICWINTWGESPAEMPVGGYKHSGIGRENGVMTLQSYTQVK 477


Lambda     K      H
   0.317    0.135    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 553
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 480
Length of database: 490
Length adjustment: 34
Effective length of query: 446
Effective length of database: 456
Effective search space:   203376
Effective search space used:   203376
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory