Alignments for a candidate for puo in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for puo in Escherichia coli BW25113

Align Primary amine oxidase 1; AtAO1; EC 1.4.3.21 (characterized)
to candidate 15508 b1386 tyramine oxidase, copper-requiring (NCBI)

Query= SwissProt::O23349
         (650 letters)



>FitnessBrowser__Keio:15508
          Length = 757

 Score =  282 bits (722), Expect = 3e-80
 Identities = 199/650 (30%), Positives = 298/650 (45%), Gaps = 44/650 (6%)

Query: 23  HPLDPLTPQEINKTSFIVKKSHLGNLKDLT-FHYLDLEEPNKSHVLQWLSPNPSKKPPPP 81
           HPL+ LT  EI +   IVK S   + K  T F  + L  P+K  V  +   N   KP   
Sbjct: 124 HPLNALTADEIKQAVEIVKAS--ADFKPNTRFTEISLLPPDKEAVWAFALEN---KPVDQ 178

Query: 82  RRRSFVVVRAGGQTYELIIDLTTSKIASSR-IYTGHGF---PSFTFIELFKASKLPLTYP 137
            R++ V++  G    E ++DL  +K+ S + I   HG      F  ++    +       
Sbjct: 179 PRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVLLDDFASVQNIINNSEEFAAA 238

Query: 138 PFKKSILDRSLNISEVSCIPFTVGWY----GETTTRRELKASCFYRDGSVNVFTRPIEGI 193
             K+ I D      +V   P TVG++    G     R LK   +   G  N +  PIE +
Sbjct: 239 VKKRGITDAK----KVITTPLTVGYFDGKDGLKQDARLLKVISYLDVGDGNYWAHPIENL 294

Query: 194 TVTIDVDSMQVIKYSDRFRKPIPDKEGNDFRTKHRPFPF-----FCNVSDTGFKILGNRV 248
              +D++  +++K  +    P+P      F  + R  P              + I G+ +
Sbjct: 295 VAVVDLEQKKIVKIEEGPVVPVP-MTARPFDGRDRVAPAVKPMQIIEPEGKNYTITGDMI 353

Query: 249 KWANWKFHVGFTARAGVTISTASVLDPRTKRFRRVMYRGHVSETFVPYMDPTYEWYYRTF 308
            W NW FH+   +R G  IST +  D  TKR  +VMY G +    VPY DP   WY++ +
Sbjct: 354 HWRNWDFHLSMNSRVGPMISTVTYNDNGTKR--KVMYEGSLGGMIVPYGDPDIGWYFKAY 411

Query: 309 MDIGEFGFGRSAVNLQPLIDCPQNAAFLDGHVAGPDGTAQKMTNVMCVFEKNGYGASFRH 368
           +D G++G G     +    D P NA  L+  +A   G   ++   + VFE+   G  ++H
Sbjct: 412 LDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVPMEIPRAIAVFERYA-GPEYKH 470

Query: 369 TEINVPGQVITSGEAEISLVVRMVATLGNYDYIVDWEFKKNGAIRVGVDLTGVLEVKAT- 427
            E+  P       E    LVVR ++T+GNYDYI DW F +NG I +    TG+  VK   
Sbjct: 471 QEMGQPNVSTERRE----LVVRWISTVGNYDYIFDWIFHENGTIGIDAGATGIEAVKGVK 526

Query: 428 SYTSNDQITENV--YGTLVAKNTIAVNHDHYLTYYLDLDVDGNGNSLVKAKLKTVRVTEV 485
           + T +D+  ++   YGTL+  N +   H H   + LDLDVDG  NSLV        V + 
Sbjct: 527 AKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDVDGENNSLVAMD----PVVKP 582

Query: 486 NKTSSRRKSYWTVVKETAKTEADGRVRLGSDPVELLIVNPNKKTKIGNTVGYRLIP---- 541
           N     R S   V +     E D   +     + LL  NPNK+ ++GN V Y++IP    
Sbjct: 583 NTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLS-NPNKENRMGNPVSYQIIPYAGG 641

Query: 542 -EHLQATSLLTDDDYPELRAGYTKYPVWVTAYDRSERWAGGFYSDRSRGDDGLAVWSSRN 600
              +   +    D++   R  +    +WVT Y   ER+  G Y +RS  D GL  +S  N
Sbjct: 642 THPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEGKYPNRSTHDTGLGQYSKDN 701

Query: 601 REIENKDIVMWYNVGFHHIPYQEDFPVMPTLHGGFTLRPSNFFDNDPLIG 650
             ++N D V+W   G  H+   E++P+MPT      L+P NFFD  P +G
Sbjct: 702 ESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKPWNFFDETPTLG 751


Lambda     K      H
   0.320    0.138    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1240
Number of extensions: 60
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 650
Length of database: 757
Length adjustment: 39
Effective length of query: 611
Effective length of database: 718
Effective search space:   438698
Effective search space used:   438698
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources