Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate 16258 b2149 fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI)
Query= TCDB::Q7BSH4 (512 letters) >FitnessBrowser__Keio:16258 Length = 506 Score = 375 bits (963), Expect = e-108 Identities = 200/507 (39%), Positives = 323/507 (63%), Gaps = 11/507 (2%) Query: 9 VTDSKTGDAPAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTG 68 V+ + +LEM GI++ FPGVKALDNV++ + P ++ AL+GENGAGKSTL+K L G Sbjct: 2 VSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFG 61 Query: 69 IYRPNEGEILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRT 128 IY+ + G IL G+ F SA+ A++ G++ +HQE L + +V +N++LG P T+ Sbjct: 62 IYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYP-TKGMF 120 Query: 129 IDWQTMNSRSKALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAAL 188 +D M +KA+ L+ +IDP R+ LS++Q ++ IA+A S A+IVIMDEPT++L Sbjct: 121 VDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSL 180 Query: 189 SRKEIDDLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRR---SRRPVRGVSRKT 245 + KE++ LF I+R LKE+G I++ISHK +E++++ D+ V + P+ G++ Sbjct: 181 TEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLT--- 237 Query: 246 PQDEIVRMMVGRDVENVFPKIDVAIGGPVLEIRNYS--HRTEFRDISFTLRKGEILGVYG 303 D+I+ MMVGR + FP + G +LE+RN + + RD+SF L KGEILG+ G Sbjct: 238 -MDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQPSIRDVSFDLHKGEILGIAG 296 Query: 304 LIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPM 363 L+GA R+++ ++LFGI + +G + L G++I H+ +AI G V EER G+ + Sbjct: 297 LVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYL 356 Query: 364 PIFQNMTLPSLARTSRR-GFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVV 422 I N + ++ + G L + + + + + ++ +G+LSGGNQQKV+ Sbjct: 357 DIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVI 416 Query: 423 IGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRV 482 IG+WL T P++++LDEPT+GID+G+K ++ I+ELA +G II++SSE+PE++G++DR+ Sbjct: 417 IGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRI 476 Query: 483 LVMKEGLSAGIFERAELSPEALVRAAT 509 LVM GL +GI + + ++R A+ Sbjct: 477 LVMSNGLVSGIVDTKTTTQNEILRLAS 503 Score = 81.6 bits (200), Expect = 6e-20 Identities = 62/226 (27%), Positives = 107/226 (47%), Gaps = 8/226 (3%) Query: 288 DISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGI 347 +++ +R I + G GAG+S L + LFGI + SG ++ +G+EI HS ++A+ GI Sbjct: 31 NVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGI 90 Query: 348 VYVPEERGRHGLALPMPIFQNMTLPSLARTSRRG-FLRAANEFALARKYAERLDLRAAAL 406 V +E L L + NM L R +G F+ + + + LD+ Sbjct: 91 SMVHQELN---LVLQRSVMDNMWL---GRYPTKGMFVDQDKMYRETKAIFDELDIDIDPR 144 Query: 407 SVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSII 466 + VGTLS Q + I K + K++I+DEPT + + I +L G I+ Sbjct: 145 A-RVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIV 203 Query: 467 MVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATGNA 512 +S ++ EI + D V V+++G A L+ + ++ G + Sbjct: 204 YISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVGRS 249 Lambda K H 0.320 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 605 Number of extensions: 32 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 512 Length of database: 506 Length adjustment: 34 Effective length of query: 478 Effective length of database: 472 Effective search space: 225616 Effective search space used: 225616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory