Alignments for a candidate for rhaT' in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Escherichia coli BW25113

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate 17628 b3567 fused D-xylose transporter subunits of ABC superfamily: ATP-binding components (NCBI)

Query= TCDB::Q7BSH4
         (512 letters)



>FitnessBrowser__Keio:17628
          Length = 513

 Score =  342 bits (876), Expect = 2e-98
 Identities = 196/502 (39%), Positives = 305/502 (60%), Gaps = 13/502 (2%)

Query: 18  PAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPN--EG 75
           P +LEM+ I++ F  VKA+DNV + L+ G + +L GENG+GKSTL+K+L GIY     EG
Sbjct: 2   PYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKSTLMKVLCGIYPHGSYEG 61

Query: 76  EILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMN 135
           EI+  G     +  +     G+  IHQE  L  ELTV ENIFLG+   T    +D+  M 
Sbjct: 62  EIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENIFLGNEI-THNGIMDYDLMT 120

Query: 136 SRSKALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDD 195
            R + LL  +  +I P  R+ DL + Q+ LV IA+AL+ + R++I+DEPTA+L+ +E   
Sbjct: 121 LRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQVRLLILDEPTASLTEQETSI 180

Query: 196 LFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIVRMMV 255
           L  I+R L++ G A ++ISHK +E+  I+D   V         R  +  + +D+I+ MMV
Sbjct: 181 LLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDGQHIGTRDAAGMS-EDDIITMMV 239

Query: 256 GRDVENVFPKIDVAIGGPVLEIRNYS-------HRTEFRDISFTLRKGEILGVYGLIGAG 308
           GR++  ++P      G  +L I + +       H     D+SF+L++GEILG+ GL+GAG
Sbjct: 240 GRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKRVNDVSFSLKRGEILGIAGLVGAG 299

Query: 309 RSELSQSLFGITK-PLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQ 367
           R+E  Q LFG+      GK+ ++G+++ I + Q AI  GI  VPE+R R G+   M + +
Sbjct: 300 RTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQAIAQGIAMVPEDRKRDGIVPVMAVGK 359

Query: 368 NMTLPSLAR-TSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKW 426
           N+TL +L + T     L  A E     +  ++L ++ ++  + +G LSGGNQQK ++ + 
Sbjct: 360 NITLAALNKFTGGISQLDDAAEQKCILESIQQLKVKTSSPDLAIGRLSGGNQQKAILARC 419

Query: 427 LATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMK 486
           L   P+++ILDEPT+GIDIG+K  ++  I++L  +G+++I++SSELPE++G+SDRVLVM 
Sbjct: 420 LLLNPRILILDEPTRGIDIGAKYEIYKLINQLVQQGIAVIVISSELPEVLGLSDRVLVMH 479

Query: 487 EGLSAGIFERAELSPEALVRAA 508
           EG          L+ E ++ AA
Sbjct: 480 EGKLKANLINHNLTQEQVMEAA 501



 Score = 74.7 bits (182), Expect = 7e-18
 Identities = 62/229 (27%), Positives = 111/229 (48%), Gaps = 16/229 (6%)

Query: 288 DISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLS--GKMVLEGQEITIHSPQDAIRA 345
           ++   L  GEI+ + G  G+G+S L + L GI    S  G+++  G+EI     +D  R 
Sbjct: 22  NVCLRLNAGEIVSLCGENGSGKSTLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERK 81

Query: 346 GIVYVPEERGRHGLALPMPIFQNMTLPSLARTSRRGFLRAANEFALARKYAERLDLRAAA 405
           GI  + +E     L   + + +N+ L +    +  G +    ++ L     ++L L   +
Sbjct: 82  GIAIIHQELA---LVKELTVLENIFLGN--EITHNGIM----DYDLMTLRCQKL-LAQVS 131

Query: 406 LSVP----VGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAE 461
           LS+     VG L  G QQ V I K L    +++ILDEPT  +     + +   I +L   
Sbjct: 132 LSISPDTRVGDLGLGQQQLVEIAKALNKQVRLLILDEPTASLTEQETSILLDIIRDLQQH 191

Query: 462 GLSIIMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATG 510
           G++ I +S +L E+  +SD + V+++G   G  + A +S + ++    G
Sbjct: 192 GIACIYISHKLNEVKAISDTICVIRDGQHIGTRDAAGMSEDDIITMMVG 240


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 580
Number of extensions: 34
Number of successful extensions: 12
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 513
Length adjustment: 35
Effective length of query: 477
Effective length of database: 478
Effective search space:   228006
Effective search space used:   228006
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources