GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fru2-IIB in Escherichia coli BW25113

Align PTS system, fructose-specific, IIB component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized)
to candidate 17939 b3899 PTS system, fructose-like enzyme IIBC component (VIMSS)

Query= TCDB::Q3JZE2
         (103 letters)



>FitnessBrowser__Keio:17939
          Length = 483

 Score = 93.6 bits (231), Expect = 3e-24
 Identities = 44/99 (44%), Positives = 71/99 (71%), Gaps = 2/99 (2%)

Query: 1   MKIVGVAACTVGIAHTYIAQEKLENASKAAGHDVKIETQGTIGIENQLTSEEIAAADIVL 60
           ++IV +  C  GIAHTY+  E LE  +++ GH +K+ETQG+ G+EN+L+SEEIAAAD V+
Sbjct: 5   LRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVI 64

Query: 61  LAVDVKIAGEE--RFEGKKVVKVPTEVAIKSPNKLIAKL 97
           LA    ++G++  RF GKKV ++    A+K+ +++ ++L
Sbjct: 65  LATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSEL 103


Lambda     K      H
   0.311    0.128    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 86
Number of extensions: 3
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 103
Length of database: 483
Length adjustment: 22
Effective length of query: 81
Effective length of database: 461
Effective search space:    37341
Effective search space used:    37341
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory