GapMind for catabolism of small carbon sources

 

D-sorbitol (glucitol) catabolism in Escherichia coli BW25113

Best path

srlA, srlB, srlE, srlD

Also see fitness data for the top candidates

Rules

Overview: D-sorbitol is also known as D-glucitol. D-sorbitol degradation in GapMind is based on MetaCyc pathways D-sorbitol degradation I (via sorbitol dehydrogenase, link) and pathway II (via sorbitol-6-phosphate 2-dehydrogenase, link).

12 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
srlA PTS system for sorbitol SrlABE, EII-C2 component SrlA b2702
srlB PTS system for sorbitol SrlABE, EII-A component SrlB b2704
srlE PTS system for sorbitol SrlABE, EII-BC1 component SrlE b2703
srlD sorbitol 6-phosphate 2-dehydrogenase b2705 b1093
Alternative steps:
mtlA PTS system for polyols, EII-CBA components b3599 b2933
mtlE ABC transporter for polyols MtlEFGK, substrate-binding component MtlE
mtlF ABC transporter for polyols MtlEFGK, permease component MtlF b1311
mtlG ABC transporter for polyols MtlEFGK, permease component MtlG b4032
mtlK ABC transporter for polyols MtlEFGK, permease component MtlK b4035 b3450
scrK fructokinase b0394 b1119
sdh sorbitol dehydrogenase b1774 b1542
SOT sorbitol:H+ co-transporter SOT1 or SOT2 b2943 b2841

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory